Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhiziinfection

BMC Plant Biology

Research Article

Ricardo V Abdelnoor¹  Renata Stolf¹  Francismar C Marcelino-Guimarães¹  Mayra CCG De Carvalho¹  Atílio P Castagnaro²  Gastón Westergaard³  Estefania Mancini³  Maria Helena Bodanese-Zanettini⁴  Caroline Cabreira⁴  Beatriz Wiebke-Strohm⁴  Marina B Osorio⁴  Milena S Homrich⁴  Lauro Bücker-Neto⁴  Andreia Carina Turchetto-Zolet⁴  Márcia Margis-Pinheiro⁴  Marta Bencke-Malato⁴  Ricardo LM Weber⁴ 

[1] Empresa Brasileira de Pesquisa Agropecuária (Embrapa Soja), Londrina, Brazil;Estación Experimental Agroindustrial Obispo Colombres (EEAOC), Tucumán, Argentina;Instituto de Agrobiotecnologia Rosario SA, Rosario, Argentina;Programa de Pós-Graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil;

关键词: Glycine max;    Genetic transformation;    Fungus resistance;    Transcription factors;    Asian Soybean Rust;    Functional analysis;   

DOI  :  10.1186/s12870-014-0236-0

 received in 2014-08-14, accepted in 2014-08-29,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundMany previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified.ResultsAs a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants.ConclusionsThe present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants.

【 授权许可】

Unknown   
© Bencke-Malato et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.