A method for culturing Gram-negative skin microbiota

BMC Microbiology

Methodology Article

Kelli W. Williams¹  Sandip K. Datta¹  Ian A. Myles¹  Jensen D. Reckhow¹  Inka Sastalla¹  Karen M. Frank² 

[1] Bacterial Pathogenesis Unit, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA;Department of Laboratory Medicine, National Institutes of Health Clinical Center, Bethesda, MD, USA;

关键词: Bacteriology;    Skin;    Microbiome;    Culture techniques;   

DOI  :  10.1186/s12866-016-0684-9

 received in 2015-11-25, accepted in 2016-03-29,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundCommensal Gram-negative (CGN) microbiota have been identified on human skin by DNA sequencing; however, methods to reliably culture viable Gram-negative skin organisms have not been previously described.ResultsThrough the use of selective antibiotics and minimal media we developed methods to culture CGN from skin swabs. We identified several previously uncharacterized CGN at the species level by optimizing growth conditions and limiting the inhibitory effects of nutrient shock, temperature, and bacterial competition, factors that may have previously limited CGN isolation from skin cultures.ConclusionsOur protocol will permit future functional studies on the influences of CGN on skin homeostasis and disease.

【 授权许可】

CC BY   
© Myles et al. 2016