Coordinate and synergistic effects of extensive treadmill exercise and ovariectomy on articular cartilage degeneration

BMC Musculoskeletal Disorders

Research Article

Ichiro Sekiya¹  Kunikazu Tsuji²  Kazumasa Miyatake³  Miyoko Ojima³  Yu Matsukura³  Takeshi Muneta³  Jun Yamada³  Kahaer Abula³ 

[1] Center for Stem Cell and Regenerative Medicine, Tokyo Medical and Dental University, 113-8519, Tokyo, Japan;Department of Cartilage Regeneration, Tokyo Medical and Dental University, 1-5-45 Yushima, 113-8519, Bunkyo-ku, Tokyo, Japan;Department of Joint Surgery and Sports Medicine, Tokyo Medical and Dental University, 113-8519, Tokyo, Japan;

关键词: Ovariectomy;    Mechanical stress;    Forced running;    Joint inflammation;    Naturally occurring osteoarthritis model;   

DOI  :  10.1186/s12891-016-1094-8

 received in 2016-03-04, accepted in 2016-05-24,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundAlthough osteoarthritis (OA) is a multifactorial disease, little has been reported regarding the cooperative interaction among these factors on cartilage metabolism. Here we examined the synergistic effect of ovariectomy (OVX) and excessive mechanical stress (forced running) on articular cartilage homeostasis in a mouse model resembling a human postmenopausal condition.MethodsMice were randomly divided into four groups, I: Sham, II: OVX, III: Sham and forced running (60 km in 6 weeks), and IV: OVX and forced running. Histological and immunohistochemical analyses were performed to evaluate the degeneration of articular cartilage and synovitis in the knee joint. Morphological changes of subchondral bone were analyzed by micro-CT.ResultsMicro-CT analyses showed significant loss of metaphyseal trabecular bone volume/tissue volume (BV/TV) after OVX as described previously. Forced running increased the trabecular BV/TV in all mice. In the epiphyseal region, no visible alteration in bone morphology or osteophyte formation was observed in any of the four groups. Histological analysis revealed that OVX or forced running respectively had subtle effects on cartilage degeneration. However, the combination of OVX and forced running synergistically enhanced synovitis and articular cartilage degeneration. Although morphological changes in chondrocytes were observed during OA initiation, no signs of bone marrow edema were observed in any of the four experimental groups.ConclusionWe report the coordinate and synergistic effects of extensive treadmill exercise and ovariectomy on articular cartilage degeneration. Since no surgical procedure was performed on the knee joint directly in this model, this model is useful in addressing the molecular pathogenesis of naturally occurring OA.

【 授权许可】

CC BY   
© The Author(s). 2016