Bo-Gan-Whan regulates proliferation and migration of vascular smooth muscle cells

BMC Complementary and Alternative Medicine

Research Article

Hae Ryong Chang¹  Dae Won Lee¹  Kang Pa Lee¹  Hyuck Kim²  Won-Hwan Park²  Jai-Eun Kim³ 

[1] Department of Bio-Science, College of Natural Science, Dongguk University, Dongdae-ro 123, 38066, Gyeongju, Gyeongsangbuk-do, Republic of Korea;Department of Diagnosis, College of Korean Medicine, Dongguk University, Dongguk-Ro 32, 10326, Goyang, Gyeonggi-do, Republic of Korea;Department of Pathology, College of Korean Medicine, Dongguk University, Dongguk-Ro 32, 10326, Goyang, Gyeonggi-do, Republic of Korea;

关键词: Bo-Gan-Whan;    Vascular smooth muscle cells;    Platelet-derived growth factor-BB;    Extracellular signal-regulated kinase 1/2;    p38 mitogen-activated protein kinase;   

DOI  :  10.1186/s12906-016-1292-9

 received in 2016-02-04, accepted in 2016-08-17,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundBo-Gan-Whan (BGH), a Korean polyherbal medicine, is used as a hepatoprotective drug. It has six natural sources, and has been demonstrated to have anti-oxidative, anti-cancer, and anti-inflammatory properties; however, its effect on vascular diseases remains unclear.MethodsCell viability and proliferation assays were employed using an EZ-Cytox Cell Viability Assay Kit. Platelet-derived growth factor (PDGF)-BB-induced vascular smooth muscle cell (VSMC) migration was measured by scratch wound healing assay and Boyden chamber assay. The expression levels of the phosphorylated signaling proteins relevant to proliferation, including extracellular signal-regulated kinase (ERK) 1/2 and p38 mitogen-activated protein kinase (MAPK) were determined by western blot analysis. Chromatogram and mass analysis were employed by Ultra Performance Liquid Chromatography (UPLC) system. Cell prolife ration and migration were also explored using the PDGF-BB-induced aortic sprout assay.ResultsBGH (100–500 μg/mL) significantly inhibited the proliferation and migration of PDGF-BB-stimulated VSMCs through the reduced phosphorylation of ERK1/2 and p38 MAPK in comparison to untreated PDGF-BB-stimulated VSMC. Moreover, we identified the paeoniflorin as the major composition of BGH.ConclusionsWe suggest that BGH may have an anti-atherosclerosis effect by inhibiting the proliferation and migration of PDGF-BB-stimulated VSMCs through down-regulation of ERK1/2 and p38 MAPK phosphorylation.

【 授权许可】

CC BY   
© The Author(s). 2016