Regulation of gene expression by FSP27 in white and brown adipose tissue

BMC Genomics

Research Article

Jianli Sang¹  De Li²  Zilong Wen³  Bofu Xue⁴  Linkang Zhou⁵  Peng Li⁵  Yue Wang⁵  Yinxin Zhang⁵  Li Xu⁶ 

[1] College of Life Sciences, Beijing Normal University, Xinjiekouwai Street 19, 100875, Xichen District, Beijing, China;College of Life Sciences, Beijing Normal University, Xinjiekouwai Street 19, 100875, Xichen District, Beijing, China;Protein Science Laboratory of the Ministry of Education, School of Life Sciences, Tsinghua University, 100084, Qinghuayuan, Haidian District, Beijing, China;Department of Biochemistry, Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong;Department of Biology, Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong;Protein Science Laboratory of the Ministry of Education, School of Life Sciences, Tsinghua University, 100084, Qinghuayuan, Haidian District, Beijing, China;Protein Science Laboratory of the Ministry of Education, School of Life Sciences, Tsinghua University, 100084, Qinghuayuan, Haidian District, Beijing, China;College of Life Science and Bioengineering, Beijing Jiaotong University, Shangyuancun 3, 100044, Haidian District, Beijing, China;

关键词: Brown Adipose Tissue;    White Adipose Tissue;    Deficient Mouse;    Gene Expression Program;    Brown Adipocyte;   

DOI  :  10.1186/1471-2164-11-446

 received in 2010-01-05, accepted in 2010-07-22,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundBrown and white adipose tissues (BAT and WAT) play critical roles in controlling energy homeostasis and in the development of obesity and diabetes. The mouse Fat-Specific protein 27 (FSP27), a member of the cell death-inducing DFF45-like effector (CIDE) family, is expressed in both BAT and WAT and is associated with lipid droplets. Over-expression of FSP27 promotes lipid storage, whereas FSP27 deficient mice have improved insulin sensitivity and are resistant to diet-induced obesity. In addition, FSP27-deficient white adipocytes have reduced lipid storage, smaller lipid droplets, increased mitochondrial activity and a higher expression of several BAT-selective genes. To elucidate the molecular mechanism by which FSP27 controls lipid storage and gene expression in WAT and BAT, we systematically analyzed the gene expression profile of FSP27- deficient WAT by microarray analysis and compared the expression levels of a specific set of genes in WAT and BAT by semi-quantitative real-time PCR analysis.ResultsBAT-selective genes were significantly up-regulated, whereas WAT-selective genes were down-regulated in the WAT of FSP27- deficient mice. The expression of the BAT-selective genes was also dramatically up-regulated in the WAT of leptin/FSP27 double deficient mice. In addition, the expression levels of genes involved in multiple metabolic pathways, including oxidative phosphorylation, the TCA cycle, fatty acid synthesis and fatty acid oxidation, were increased in the FSP27- deficient WAT. In contrast, the expression levels for genes involved in extracellular matrix remodeling, the classic complement pathway and TGF-β signaling were down-regulated in the FSP27- deficient WAT. Most importantly, the expression levels of regulatory factors that determine BAT identity, such as CEBPα/β, PRDM16 and major components of the cAMP pathway, were markedly up-regulated in the WAT of FSP27- deficient mice. The expression levels of these regulatory factors were also up-regulated in leptin/FSP27 double deficient mice. Interestingly, distinct gene expression profiles were observed in the BAT of FSP27- deficient mice. Taken together, these data suggest that the WAT of FSP27- deficient mice have a gene expression profile similar to that of BAT.ConclusionsFSP27 acts as a molecular determinant that controls gene expression for a diversity of metabolic and signaling pathways and, in particular, the expression of regulatory factors, including CEBPα/β, PRDM16 and components of the cAMP signaling pathway, that control the identity of WAT and BAT.

【 授权许可】

CC BY   
© Li et al; licensee BioMed Central Ltd. 2010