Intestinal and neuronal myenteric adaptations in the small intestine induced by a high-fat diet in mice

BMC Gastroenterology

Research Article

Angelica Soares¹  Evandro José Beraldi²  Nilza Cristina Buttow²  Paulo Emílio Botura Ferreira²  Roberto Barbosa Bazotte³ 

[1] Center of Medical and Pharmaceutical Sciences, State University of the West of Paraná, R. Universitária, 1619, CEP 85819-110, Cascavel, PR, Brazil;Department of Morphological Sciences, State University of Maringá, Av. Colombo, 5790, CEP 87020-900, Maringá, PR, Brazil;Department of Pharmacology and Therapeutics, State University of Maringá, Av. Colombo, 5790, CEP 87020-900, Maringá, PR, Brazil;

关键词: Intestinal wall;    Myenteric plexus;    Myosin-V;    Neuronal nitric oxide synthase;    Vasoactive intestinal peptide;    Enteroendocrine cells;    Goblet cells;    Intraepithelial lymphocytes;   

DOI  :  10.1186/s12876-015-0228-z

 received in 2014-06-18, accepted in 2015-01-05,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe prevalence of obesity has increased at alarming rates, particularly because of the increased consumption of high-fat diets (HFDs). The influence of HFDs on intrinsic innervation and the intestinal wall has not been fully characterized. The aim of this study was to investigate the morpho-quantitative aspects of myenteric neurons and the wall of the small intestine in mice fed a HFD.MethodsSwiss mice were fed a HFD (59% kcal from fat) or standard chow (9% Kcal from fat) for 8 weeks. Segments of the duodenum, jejunum, and ileum were subjected to histological processing for morpho-quantitative examination of the intestinal wall and mucosal cells, and immunohistochemistry was performed to evaluate myenteric neurons. The data for each segment were compared between the groups using an unpaired Student’s t-test or an equivalent nonparametric test.ResultsThe HFD increased body weight and visceral fat and decreased the length of the small intestine and the circumference of the ileum. In the duodenum, the HFD increased the density of the nitrergic subpopulation and decreased the area of nitrergic neurons and vasoactive intestinal peptide (VIP) varicosities. In the jejunum, the density of the nitrergic subpopulation was increased and the neuronal areas of the general population, nitrergic subpopulation and (VIP) varicosities were reduced. In the ileum, the density of the general population and nitrergic subpopulation were increased and the neuronal areas of the general population, nitrergic subpopulation and (VIP) varicosities were reduced. The morphometric parameters of the villi, crypts, muscular layer and total wall generally increased in the duodenum and jejunum and decreased in the ileum. In the duodenum and jejunum, the HFD promoted a decreased in the proportion of intraepithelial lymphocytes. In the ileum, the proportion of intraepithelial lymphocytes and goblet cells reduced, and the enteroendocrine cells increased.ConclusionsThe high-fat diet induces changes in the myenteric innervation of the small intestine, intestinal wall and mucosal cells responsible for the secretion of hormones and maintenance of the protective intestinal barrier. The morpho-quantitative data provide a basis for further studies to clarify the influence of HFD in the motility, digestive and absorptive capacity, and intestinal barrier.

【 授权许可】

Unknown   
© Soares et al.; licensee BioMed Central. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.