Characterisation of a household norovirus outbreak occurred in Valencia (Spain)

BMC Infectious Diseases

Research Article

Jesús Rodríguez-Díaz¹  Susana Vila-Vicent¹  Noelia Carmona-Vicente¹  Manuel Fernández-Jiménez¹  Javier Buesa¹ 

[1] Department of Microbiology, School of Medicine, University of Valencia, Avda. Blasco Ibáñez 17, 46010, Valencia, Spain;

关键词: Norovirus;    Susceptibility;    Histo-blood group antigens;    FUT2;    Secretor;    Virus-like particles;    IgA;    IgG;    Binding assay;   

DOI  :  10.1186/s12879-016-1455-9

 received in 2015-11-09, accepted in 2016-03-04,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundHuman noroviruses (NoVs) are the main cause of non-bacterial gastroenteritis worldwide. Several studies have linked human susceptibility to NoVs with the expression of histo-blood group antigens (HBGAs). In January 2012, a NoV gastroenteritis outbreak affected a household in Valencia, Spain, and the personal susceptibility to NoV was investigated.MethodsTo reach this aim 8 members of the affected household were recruited for this study and their secretor status, ABO and Lewis antigens were determined. NoV-specific saliva IgA and serum IgG antibody titers were analyzed. Their capacity to block viral binding to saliva receptors was analyzed, using virus-like particles (VLPs) of the NoV GII.4 genotype, 2006b variant, and saliva from a secretor O blood type donor.ResultsThe most relevant finding was that an asymptomatic non-secretor individual shed NoVs in his stools. Interestingly, anti-NoV IgA antibody titers in saliva from secretor and non-secretor individuals showed no differences. On the contrary, high titers of NoV-specific IgG antibody were found in both convalescent sera and in sera collected 1 year post-infection, but only from secretor individuals. NoV GII.4-2006b VLP binding to receptors present in the saliva was efficiently blocked only by sera from secretor positive individuals.ConclusionsDespite the small number of individuals involved in this outbreak, this study reinforces the idea that susceptibility to human NoV is both dependent on the HBGA profile of the individuals as well as on the viral genotype and variant. We also show that the immunity to NoV lasts for at least 1 year after infection, demonstrating that symptomatic infections strongly stimulate immune responses.

【 授权许可】

CC BY   
© Carmona-Vicente et al. 2016