Acute dengue virus 2 infection in Gabonese patients is associated with an early innate immune response, including strong interferon alpha production

BMC Infectious Diseases

Research Article

Cindy Padilla¹  Dieudonné Nkoghe¹  Nadia Wauquier²  Eric M Leroy³  Pierre Becquart³  Marc Souris⁴  Angélique Ndjoyi-Mbiguino⁵ 

[1] Centre International de Recherches Médicales de Franceville, BP 769, Franceville, Gabon;Centre International de Recherches Médicales de Franceville, BP 769, Franceville, Gabon;Institut National de la Santé et de la Recherche Médicale UMR-S 945, Laboratoire d'Immunité et Infection, Université Pierre et Marie Curie Paris 6, Paris, France;Centre International de Recherches Médicales de Franceville, BP 769, Franceville, Gabon;UMR 264 MIVEGEC, Institut de Recherche pour le Développement (IRD)/Centre National pour la Recherche Scientifique/Université Montpellier I, Montpellier, France;UMR 190 Emergence des pathologies virales/IRD, Marseille, France;CVVD, Faculty of Science, Mahidol University, Salaya, Thailand;Université des Sciences de la Santé, Libreville, Gabon;

关键词: Dengue Virus;    Dengue Fever;    Dengue Hemorrhagic Fever;    Dengue Infection;    Chikungunya;   

DOI  :  10.1186/1471-2334-10-356

 received in 2010-05-03, accepted in 2010-12-17,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundDengue is now a leading cause of morbidity and mortality throughout the tropics. We conducted the first ex vivo study of dengue fever (DF) in African patients infected during the first Gabonese dengue virus 2 (DENV-2) outbreak in 2007, in order to investigate cytokine production, including the antiviral cytokine IFN-α, reported to be a potent inhibitor of DENV replication in vitro.MethodsLevels of 50 cytokines, chemokines and growth factors were measured in plasma from 36 patients with DENV-2 infection, and in uninfected controls, using Luminex multiplex technology. The results were interpreted according to the day of sampling after symptom onset. PBMC from six patients were also studied for T lymphocyte cell surface marker expression by flow cytometry.ResultsAcute DENV-2 infection elicited high levels of several pro-inflammatory cytokines (IL-6 and IL-17), chemokines (MIF, RANTES, IP-10 and MCP-1) and growth factors (G-CSF, GM-CSF and VEGF-A). We also observed high levels of IFN-α for the first time in adult DF patients, and CD4+ and CD8+ T cell activation at symptom onset.ConclusionAcute DENV-2 infection in African patients elicits a strong innate response involving IFN-α production, as well as an adaptive immune response.

【 授权许可】

CC BY   
© Becquart et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.