Analysis of the lipid body proteome of the oleaginous alga Lobosphaera incisa

BMC Plant Biology

Research Article

Inna Khozin-Goldberg¹  Olivier Vallon²  Nicolas J. Tourasse³  Till Ischebeck⁴  Jennifer Popko⁴  Heike Siegler⁴  Ivo Feussner⁵  Oliver Valerius⁶  Gerhard H. Braus⁷ 

[1] Ben-Gurion University of the Negev, Microalgal Biotechnology Laboratory, Beer-Sheva, Israel;UMR7141, CNRS/Université Pierre et Marie Curie, Paris, France;UMR7141, CNRS/Université Pierre et Marie Curie, Paris, France;Present address: Laboratoire ARNA, INSERM U1212, CNRS UMR5320, Université Bordeaux 2; Institut Européen de Chimie et Biologie (IECB), 2 rue Robert Escarpit, 33607, Pessac, France;University of Goettingen, Albrecht-von-Haller-Institute for Plant Sciences, Department of Plant Biochemistry, Goettingen, Germany;University of Goettingen, Albrecht-von-Haller-Institute for Plant Sciences, Department of Plant Biochemistry, Goettingen, Germany;University of Goettingen, Goettingen Center for Molecular Biosciences (GZMB), Goettingen, Germany;University of Goettingen, International Center for Advanced Studies of Energy Conversion (ICASEC), Goettingen, Germany;University of Goettingen, Institute for Microbiology and Genetics, Department of Molecular Microbiology and Genetics, Goettingen, Germany;University of Goettingen, Institute for Microbiology and Genetics, Department of Molecular Microbiology and Genetics, Goettingen, Germany;University of Goettingen, Goettingen Center for Molecular Biosciences (GZMB), Goettingen, Germany;

关键词: Lipid bodies;    Lipid droplets;    Lobosphaera incisa;    Nitrogen starvation;    Oil bodies;    Proteome;    Parietochloris;    TAG lipase;   

DOI  :  10.1186/s12870-017-1042-2

 received in 2017-02-06, accepted in 2017-05-22,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundLobosphaera incisa (L. incisa) is an oleaginous microalga that stores triacylglycerol (TAG) rich in arachidonic acid in lipid bodies (LBs). This organelle is gaining attention in algal research, since evidence is accumulating that proteins attached to its surface fulfill important functions in TAG storage and metabolism.ResultsHere, the composition of the LB proteome in L incisa was investigated by comparing different cell fractions in a semiquantitative proteomics approach. After applying stringent filters to the proteomics data in order to remove contaminating proteins from the list of possible LB proteins (LBPs), heterologous expression of candidate proteins in tobacco pollen tubes, allowed us to confirm 3 true LBPs: A member of the algal Major Lipid Droplet Protein family, a small protein of unknown function and a putative lipase. In addition, a TAG lipase that belongs to the SUGAR DEPENDENT 1 family of TAG lipases known from oilseed plants was identified. Its activity was verified by functional complementation of an Arabidopsis thaliana mutant lacking the major seed TAG lipases.ConclusionsHere we describe 3 LBPs as well as a TAG lipase from the oleaginous microalga L. incisa and discuss their possible involvement in LB metabolism. This study highlights the importance of filtering LB proteome datasets and verifying the subcellular localization one by one, so that contaminating proteins can be recognized as such. Our dataset can serve as a valuable resource in the identification of additional LBPs, shedding more light on the intriguing roles of LBs in microalgae.

【 授权许可】

CC BY   
© The Author(s). 2017