Response of the goat mammary gland to infection with Staphylococcus aureus revealed by gene expression profiling in milk somatic and white blood cells

BMC Genomics

Research Article

Giuliano Pisoni¹  Paola Modesto²  Paolo Moroni³  Rossana Capoferri⁴  Rachel Rupp⁵  Hugues Caillat⁵  Paola Cremonesi⁶  Bianca Castiglioni⁶  Alessandra Stella⁷  Marcello Del Corvo⁸  John L Williams⁸  Francesco Strozzi⁸ 

[1] Dipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare, Università degli Studi di Milano, via Celoria 10, 20133, Milano, Italy;Dipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare, Università degli Studi di Milano, via Celoria 10, 20133, Milano, Italy;Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, via Bologna 148, Torino, Italy;Dipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare, Università degli Studi di Milano, via Celoria 10, 20133, Milano, Italy;Quality Milk Production Services, Cornell University, 240 Farrier Road, 14853, Ithaca, NY, USA;IDRA-LAB Istituto Sperimentale Italiano “L. Spallanzani”, via Einstein, 26900, Lodi, Italy;INRA, UR631, Station d’Amélioration Génétique des Animaux, F-31326, Castanet-Tolosan, France;Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, via Einstein, 26900, Lodi, Italy;Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, via Einstein, 26900, Lodi, Italy;Parco Tecnologico Padano, via Einstein, 26900, Lodi, Italy;Parco Tecnologico Padano, via Einstein, 26900, Lodi, Italy;

关键词: Mammary Gland;    Milk Sample;    Mastitis;    Mean Platelet Volume;    Somatic Cell Score;   

DOI  :  10.1186/1471-2164-13-540

 received in 2012-03-05, accepted in 2012-09-28,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundS. aureus is one of the main pathogens responsible for the intra-mammary infection in dairy ruminants. Although much work has been carried out to understand the complex physiological and cellular events that occur in the mammary gland in response to S. aureus, the protective mechanisms are still poorly understood. The objectives of the present study were to investigate gene expression during the early response of the goat mammary gland to an experimental challenge with S. aureus, in order to better understand the local and systemic response and to compare them in two divergent lines of goat selected for high and low milk somatic cell scores.ResultsNo differences in gene expression were found between high and low SCS (Somatic Cells Score) selection lines. Analysing the two groups together, an expression of 300 genes were found to change from T0 before infection, and T4 at 24 hours and T5 at 30 hours following challenge. In blood derived white blood cells 8 genes showed increased expression between T0 and T5 and 1 gene has reduced expression. The genes showing the greatest increase in expression following challenge (5.65 to 3.16 fold change) play an important role in (i) immune and inflammatory response (NFKB1, TNFAIP6, BASP1, IRF1, PLEK, BATF3); (ii) the regulation of innate resistance to pathogens (PTX3); and (iii) the regulation of cell metabolism (CYTH4, SLC2A6, ARG2). The genes with reduced expression (−1.5 to −2.5 fold) included genes involved in (i) lipid metabolism (ABCG2, FASN), (ii) chemokine, cytokine and intracellular signalling (SPPI), and (iii) cell cytoskeleton and extracellular matrix (KRT19).ConclusionsAnalysis of genes with differential expression following infection showed an inverse relationship between immune response and lipid metabolism in the early response of the mammary gland to the S. aureus challenge. PTX3 showed a large change in expression in both milk and blood, and is therefore a candidate for further studies on immune response associated with mastitis.

【 授权许可】

Unknown   
© Cremonesi et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.