Hepatoprotective effect of ethanolic extract of Curcuma longa on thioacetamide induced liver cirrhosis in rats

BMC Complementary and Alternative Medicine

Research Article

Ahmed S AlRashdi¹  Salim S Alkiyumi¹  Mahmood Ameen Abdulla¹  Suzy M Salama¹  Shahram Golbabapour²  Salmah Ismail³ 

[1]Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603, Kuala, Lumpur, Malaysia

[2]Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603, Kuala, Lumpur, Malaysia

[3]Institute of Biological science, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia

[4]Institute of Biological science, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia

关键词: Curcuma longa;    Antioxidant enzymes;    Cytochrome P450 2E1 (CYP2E1);    Histology;    Oxidative stress;    Immunohistochemistry;   

DOI  :  10.1186/1472-6882-13-56

 received in 2012-07-24, accepted in 2013-02-20,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundHepatology research has focused on developing traditional therapies as pharmacological medicines to treat liver cirrhosis. Thus, this study evaluated mechanisms of the hepatoprotective activity of Curcuma longa rhizome ethanolic extract (CLRE) on thioacetamide-induced liver cirrhosis in rats.MethodsThe hepatoprotective effect of CLRE was measured in a rat model of thioacetamide-induced liver cirrhosis over 8 weeks. Hepatic cytochrome P450 2E1 and serum levels of TGF-β1 and TNF-α were evaluated. Oxidative stress was measured by malondialdehyde, urinary 8-hydroxyguanosine and nitrotyrosine levels. The protective activity of CLRE free-radical scavenging mechanisms were evaluated through antioxidant enzymes. Protein expression of pro-apoptotic Bax and anti-apoptotic Bcl-2 proteins in animal blood sera was studied and confirmed by immunohistochemistry of Bax, Bcl2 proteins and proliferating cell nuclear antigen.ResultsHistopathology, immunohistochemistry and liver biochemistry were significantly lower in the Curcuma longa-treated groups compared with controls. CLRE induced apoptosis, inhibited hepatocytes proliferation but had no effect on hepatic CYP2E1 levels.ConclusionThe progression of liver cirrhosis could be inhibited by the antioxidant and anti-inflammatory activities of CLRE and the normal status of the liver could be preserved.

【 授权许可】

Unknown   
© Salama et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.