miRNeye: a microRNA expression atlas of the mouse eye

BMC Genomics

Research Article

Marchesa Bilio¹  Giampiero Lago¹  Sandro Banfi¹  Ivana Peluso¹  Marianthi Karali¹  Vincenzo A Gennarino²  Roberta Verde³  Pascal Dollé⁴ 

[1] Telethon Institute for Genetics and Medicine, Via P. Castellino 111, 80131, Naples, Italy;Telethon Institute for Genetics and Medicine, Via P. Castellino 111, 80131, Naples, Italy;Department of Molecular and Human Genetics, Baylor College of Medicine, 77030, Houston, Texas, USA;Telethon Institute for Genetics and Medicine, Via P. Castellino 111, 80131, Naples, Italy;ICB (Istituto di Chimica Biomolecolare), CNR, Via Campi Flegrei 34, 80078, Naples, Italy;UMR 7104 CNRS; U 964 INSERM, IGBMC (Institute de Génétique et de Biologie Moléculaire et Cellulaire), BP 10142, 67404, Illkirch, France;

关键词: Retinal Pigment Epithelium;    miRNA Expression;    Lock Nucleic Acid;    Ocular Tissue;    Neural Retina;   

DOI  :  10.1186/1471-2164-11-715

 received in 2010-07-27, accepted in 2010-12-20,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundMicroRNAs (miRNAs) are key regulators of biological processes. To define miRNA function in the eye, it is essential to determine a high-resolution profile of their spatial and temporal distribution.ResultsIn this report, we present the first comprehensive survey of miRNA expression in ocular tissues, using both microarray and RNA in situ hybridization (ISH) procedures. We initially determined the expression profiles of miRNAs in the retina, lens, cornea and retinal pigment epithelium of the adult mouse eye by microarray. Each tissue exhibited notably distinct miRNA enrichment patterns and cluster analysis identified groups of miRNAs that showed predominant expression in specific ocular tissues or combinations of them. Next, we performed RNA ISH for over 220 miRNAs, including those showing the highest expression levels by microarray, and generated a high-resolution expression atlas of miRNAs in the developing and adult wild-type mouse eye, which is accessible in the form of a publicly available web database. We found that 122 miRNAs displayed restricted expression domains in the eye at different developmental stages, with the majority of them expressed in one or more cell layers of the neural retina.ConclusionsThis analysis revealed miRNAs with differential expression in ocular tissues and provided a detailed atlas of their tissue-specific distribution during development of the murine eye. The combination of the two approaches offers a valuable resource to decipher the contributions of specific miRNAs and miRNA clusters to the development of distinct ocular structures.

【 授权许可】

Unknown   
© Karali et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.