MicroRNA profiling in the left atrium in patients with non-valvular paroxysmal atrial fibrillation

BMC Cardiovascular Disorders

Research Article

Meng Xin¹  Jun Wang¹  Jiangang Wang¹  Yan Li¹  Shiqiu Song¹  Tiange Luo¹  Jie Han¹  Xu Meng¹  Jiahai Shi²  Changqing Xie³  Bo Yang⁴ 

[1] Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, 100029, Beijing, P.R. China;Department of Cardiothoracic Surgery, Affiliated Hospital of Nantong University, 226001, Nantong, China;Department of Internal Medicine, Vidant Medical Center, Broody School of Medicine, East Carolina University, 27834, Greenville, North Carolina, USA;University of Michigan Cardiovascular Center, University of Michigan, 48109, Ann Arbor, Michigan, USA;

关键词: Atrial Fibrillation;    Left Atrium;    Atrial Fibrillation Patient;    miRNA Expression Profile;    Left Atrial Appendage;   

DOI  :  10.1186/s12872-015-0085-2

 received in 2015-02-19, accepted in 2015-08-10,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundWe aimed to identify the miRNA expression profiles in left atrial appendage, with the intention of identifying miRNAs that were significantly associated with non-valvular paroxysmal AF.MethodsThe RNA samples were isolated from healthy controls (n = 5) and patients with atrial fibrillation (n = 8). To confirm the findings obtained by analyzing the miRNA profile, we measured the expression of selected miRNAs in the entire cohort by quantitative PCR.ResultsTen specific miRNAs were found to be differentially expressed between atrial fibrillation and healthy controls with more than a 2-fold change (P < 0.05). Consistent with the data obtained for the profile, expression levels of miRNA-155, miRNA-146b-5p and miRNA-19b were significantly increased in patients with atrial fibrillation. Interestingly, levels of miRNA-146b-5p and miRNA-155, which are known to be associated with inflammation, were independently and positively associated with left atrium dimension, atrial fibrillation duration and high sensitivity C-reactive protein levels. By using four Databases (TargetScan, miRanda, Starbase Clip-seq and miRDB) to perform target gene prediction, there were four genes were related to the inflammatory response and fibrosis, and three others encoding cardiac ion channel proteins. As a result of TaqMan qPCR and Western analysis, the relative mRNA and protein expression level of three target genes (DIER-1, TIMP-4 and CACNA1C) were significantly lower in the atrial fibrillation group than that in the healthy control group.ConclusionsExpression of inflammation-associated miRNAs is significantly up-regulated in the left atrial appendage of patients with non-valvular paroxysmal atrial fibrillation, which may play a significant role in electrical and structural remodeling.

【 授权许可】

CC BY   
© Wang et al. 2015