Proteomic characterization of the Rph15 barley resistance gene-mediated defence responses to leaf rust

BMC Genomics

Research Article

Letizia Bernardo¹  Luigi Cattivelli¹  Giampiero Valè²  Alfredo Simone Negri³  Luca Espen³  Bhakti Prinsi³ 

[1] CRA-Consiglio per la ricerca e la sperimentazione in agricoltura, Genomics Research Centre, Via S. Protaso 302, I-29017, Fiorenzuola d’Arda, PC, Italy;CRA-Consiglio per la ricerca e la sperimentazione in agricoltura, Genomics Research Centre, Via S. Protaso 302, I-29017, Fiorenzuola d’Arda, PC, Italy;CRA-Consiglio per la ricerca e la sperimentazione in agricoltura, Rice Research Unit, S.S. 11 to Torino, Km 2,5, 13100, Vercelli, Italy;Dipartimento di Scienze agrarie ambientali – Produzione – Territorio – Agroenergia (Di.S.A.A), University of Milan, via Celoria 2, I-20133, Milano, Italy;

关键词: Barley;    Leaf rust;    Resistance gene;    Rph15;    Proteomics;    Near isogenic lines;   

DOI  :  10.1186/1471-2164-13-642

 received in 2012-04-27, accepted in 2012-11-09,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundLeaf rust, caused by the biotrophic fungal pathogen Puccinia hordei, is one of the most important foliar disease of barley (Hordeum vulgare) and represents a serious threat in many production regions of the world. The leaf rust resistance gene Rph15 is of outstanding interest for resistance breeding because it confers resistance to over 350 Puccinia hordei isolates collected from around the world. Molecular and biochemical mechanisms responsible for the Rph15 effectiveness are currently not investigated. The aim of the present work was to study the Rph15-based defence responses using a proteomic approach.ResultsProtein pattern changes in response to the leaf rust pathogen infection were investigated in two barley near isogenic lines (NILs), Bowman (leaf rust susceptible) and Bowman-Rph15 (leaf rust resistant), differing for the introgression of the leaf rust resistance gene Rph15. Two infection time points, 24 hours and four days post inoculation (dpi), were analysed. No statistically significant differences were identified at the early time point, while at 4 dpi eighteen protein spots were significantly up or down regulated with a fold-change equal or higher than two in response to pathogen infection. Almost all the pathogen-responsive proteins were identified in the Bowman-Rph15 resistant NIL. Protein spots were characterized by LC-MS/MS analysis and found to be involved in photosynthesis and energy metabolism, carbohydrate metabolism, protein degradation and defence. Proteomic data were complemented by transcriptional analysis of the respective genes. The identified proteins can be related to modulation of the photosynthetic apparatus components, re-direction of the metabolism to sustain defence responses and deployment of defence proteins.ConclusionsThe identification of leaf rust infection-modulated defence responses restricted to the resistant NIL support the hypothesis that basal defence responses of Bowman, but not the Rph15 resistance gene-based ones, are suppressed or delayed by pathogen effectors to levels below the detection power of the adopted proteomic approach. Additionally, Rph15-mediated resistance processes identified mainly resides on a modulation of primary metabolism, affecting photosyntesis and carbohydrate pool.

【 授权许可】

Unknown   
© Bernardo et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.