Identification and characterization of LIM gene family in Brassica rapa

BMC Genomics

Research Article

Mi-Young Chung¹  Yong-Gu Cho²  Nasar Uddin Ahmed³  Jong-In Park³  Ill-Sup Nou³  Hee-Jeong Jung³  Senthil Kumar Thamil Arasan³  Masao Watanabe⁴ 

[1] Department of Agricultural Education, Sunchon National University, 413 Jungangno, 540-742, Suncheon, Jeonnam, Republic of Korea;Department of Crop Science, Chungbuk National University, 410 Seongbongro, 361-763, Heungdokgu, Cheongju, Republic of Korea;Department of Horticulture, Sunchon National University, 255 Jungangno, 540-950, Suncheon, Jeonnam, Republic of Korea;Laboratory of Plant Reproductive Genetics, Graduate School of Life Sciences, Tohoku University, 2-1-1, Katahira, 980-8577, Aoba-ku, Sendai, Japan;

关键词: Brassica rapa;    Characterization;    Expression analysis;    LIM;    Stress resistance;   

DOI  :  10.1186/1471-2164-15-641

 received in 2014-02-05, accepted in 2014-07-24,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundLIM (Lin-11, Isl-1 and Mec-3 domains) genes have been reported to trigger the formation of actin bundles, a major higher-order cytoskeletal assembly, in higher plants; however, the stress resistance related functions of these genes are still not well known. In this study, we collected 22 LIM genes designated as Brassica rapa LIM (BrLIM) from the Brassica database, analyzed the sequences, compared them with LIM genes of other plants and analyzed their expression after applying biotic and abiotic stresses in Chinese cabbage.ResultsUpon sequence analysis these genes were confirmed as LIM genes and found to have a high degree of homology with LIM genes of other species. These genes showed distinct clusters when compared to other recognized LIM proteins upon phylogenetic analysis. Additionally, organ specific expression of these genes was observed in Chinese cabbage plants, with BrPLIM2a, b, c, BrDAR1, BrPLIM2e, f and g only being expressed in flower buds. Furthermore, the expression of these genes (except for BrDAR1 and BrPLIM2e) was high in the early flowering stages. The remaining genes were expressed in almost all organs tested. All BrDAR genes showed higher expression in flower buds compared to other organs. These organ specific expressions were clearly correlated with the phylogenetic grouping. In addition, BrWLIM2c and BrDAR4 responded to Fusarium oxysporum f. sp. conglutinans infection, while commonly two BrDARs and eight BrLIMs responded to cold, ABA and pH (pH5, pH7 and pH9) stress treatments in Chinese cabbage plants.ConclusionTaken together, the results of this study indicate that BrLIM and BrDAR genes may be involved in resistance against biotic and abiotic stresses in Brassica.

【 授权许可】

Unknown   
© Park et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.