Intranasal immunization with plasmid DNA encoding spike protein of SARS-coronavirus/polyethylenimine nanoparticles elicits antigen-specific humoral and cellular immune responses

BMC Immunology

Research Article

Dhananjay Jere¹  Chong-Su Cho¹  Cheol-Heui Yun²  Ji-Shan Quan³  Byoung-Shik Shim⁴  Yong-Ho Park⁵  Sung-Moo Park⁶  Moon-Sik Yang⁷  Yong-Suk Jang⁷  Hyuk Chu⁸  Dong Wook Kim⁹  Man Ki Song⁹  Seung Hyun Han^1,10 

[1] Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Republic of Korea;Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Republic of Korea;Center for Agricultural Biomaterials, Seoul National University, 151-921, Seoul, Republic of Korea;Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Republic of Korea;College of Pharmacy, Yanbian University, Jilin Province, 133000, PR, China;Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, Republic of Korea;Laboratory Science Division, International Vaccine Institute, 151-818, Seoul, Republic of Korea;Department of Microbiology, College of Veterinary medicine, Seoul National University, 151-921, Seoul, Republic of Korea;Department of Microbiology, College of Veterinary medicine, Seoul National University, 151-921, Seoul, Republic of Korea;Laboratory Science Division, International Vaccine Institute, 151-818, Seoul, Republic of Korea;Division of Biological Sciences and The Institute for Molecular Biology and Genetics, Chonbuk National University, 561-756, Jeonju, Republic of Korea;Division of Zoonoses, Center for Immunology & Pathology, National Institute of Health, Korea Center for Disease Control & Prevention, 122-701, Seoul, Republic of Korea;Laboratory Science Division, International Vaccine Institute, 151-818, Seoul, Republic of Korea;Laboratory Science Division, International Vaccine Institute, 151-818, Seoul, Republic of Korea;Department of Oral Microbiology & Immunology, Dental Research Institute, and BK21 Program, School of Dentistry, Seoul National University, 110-749, Seoul, Republic of Korea;

关键词: Severe Acute Respiratory Syndrome;    Severe Acute Respiratory Syndrome;    Mucosal Immune Response;    Intracellular Cytokine Staining;    Spike Protein;   

DOI  :  10.1186/1471-2172-11-65

 received in 2010-03-29, accepted in 2010-12-31,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundImmunization with the spike protein (S) of severe acute respiratory syndrome (SARS)-coronavirus (CoV) in mice is known to produce neutralizing antibodies and to prevent the infection caused by SARS-CoV. Polyethylenimine 25K (PEI) is a cationic polymer which effectively delivers the plasmid DNA.ResultsIn the present study, the immune responses of BALB/c mice immunized via intranasal (i.n.) route with SARS DNA vaccine (pci-S) in a PEI/pci-S complex form have been examined. The size of the PEI/pci-S nanoparticles appeared to be around 194.7 ± 99.3 nm, and the expression of the S mRNA and protein was confirmed in vitro. The mice immunized with i.n. PEI/pci-S nanoparticles produced significantly (P < 0.05) higher S-specific IgG1 in the sera and mucosal secretory IgA in the lung wash than those in mice treated with pci-S alone. Compared to those in mice challenged with pci-S alone, the number of B220+ cells found in PEI/pci-S vaccinated mice was elevated. Co-stimulatory molecules (CD80 and CD86) and class II major histocompatibility complex molecules (I-Ad) were increased on CD11c+ dendritic cells in cervical lymph node from the mice after PEI/pci-S vaccination. The percentage of IFN-γ-, TNF-α- and IL-2-producing cells were higher in PEI/pci-S vaccinated mice than in control mice.ConclusionThese results showed that intranasal immunization with PEI/pci-S nanoparticles induce antigen specific humoral and cellular immune responses.

【 授权许可】

Unknown   
© Shim et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.