BMC Microbiology | |
Effects of hepatitis C virus core protein and nonstructural protein 4B on the Wnt/β-catenin pathway | |
Research Article | |
Ya-Ping Cai1  Yu-Tao Xie2  Xiao-Hua Jiang3  Jing Ren3  Tao Ma3  | |
[1] Department of Epidemiology and Health Statistics, the University of South China, 421001, Hengyang, China;Department of Infectious Diseases, Xiangya Hospital of Central South University, 410087, Changsha, China;Department of Infectious Diseases, the First Affiliated Hospital of the University of South China, 421001, Hengyang, China; | |
关键词: HCV; Core protein; NS4B; Wnt/β-catenin signaling pathway; | |
DOI : 10.1186/s12866-017-1032-4 | |
received in 2017-01-13, accepted in 2017-05-15, 发布年份 2017 | |
来源: Springer | |
【 摘 要 】
BackgroundHepatitis C virus (HCV) core protein and nonstructural protein 4B (NS4B) are potentially oncogenic. Aberrant activation of the Wnt/β-catenin signaling pathway is closely associated with hepatocarcinogenesis. We investigated the effects of HCV type 1b core protein and NS4B on Wnt/β-catenin signaling in various liver cells, and explored the molecular mechanism underlying HCV-related hepatocarcinogenesis.ResultsCompared with the empty vector control, HCV core protein and NS4B demonstrated the following characteristics in the Huh7 cells: significantly enhanced β-catenin/Tcf-dependent transcriptional activity (F = 40.87, P < 0.01); increased nuclear translocation of β-catenin (F = 165.26, P < 0.01); upregulated nuclear β-catenin, cytoplasmic β-catenin, Wnt1, c-myc, and cyclin D1 protein expression (P < 0.01); and promoted proliferation of Huh7 cells (P < 0.01 or P < 0.05). Neither protein enhanced β-catenin/Tcf-dependent transcriptional activity in the LO2 cells (F = 0.65, P > 0.05), but they did significantly enhance Wnt3a-induced β-catenin/Tcf-dependent transcriptional activity (F = 64.25, P < 0.01), and promoted the nuclear translocation of β-catenin (F = 66.54, P < 0.01) and the Wnt3a-induced proliferation of LO2 cells (P < 0.01 or P < 0.05). Moreover, activation of the Wnt/β-catenin signaling pathway was greater with the core protein than with NS4B (P < 0.01 or P < 0.05).ConclusionsHCV core protein and NS4B directly activate the Wnt/β-catenin signaling pathway in Huh7 cells and LO2 cells induced by Wnt3a. These data suggest that HCV core protein and NS4B contribute to HCV-associated hepatocellular carcinogenesis.
【 授权许可】
CC BY
© The Author(s). 2017
【 预 览 】
Files | Size | Format | View |
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RO202311099399340ZK.pdf | 8857KB | download |
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