The RootScope: a simple high-throughput screening system for quantitating gene expression dynamics in plant roots

BMC Plant Biology

Methodology Article

Erin J Kast¹  Rosalie E Lawrence¹  Nicholas J Kaplinsky¹  Christina Rabeler¹  Minh-Duyen T Nguyen¹ 

[1] Department of Biology, Swarthmore College, 19081, Swarthmore, PA, USA;

关键词: Heat shock;    Thermometer;    Thermostat;    Automated imaging;    Quantitative imaging;    Temperature;    Arabidopsis;   

DOI  :  10.1186/1471-2229-13-158

 received in 2013-05-22, accepted in 2013-10-09,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundHigh temperature stress responses are vital for plant survival. The mechanisms that plants use to sense high temperatures are only partially understood and involve multiple sensing and signaling pathways. Here we describe the development of the RootScope, an automated microscopy system for quantitating heat shock responses in plant roots.ResultsThe promoter of Hsp17.6 was used to build a Hsp17.6p:GFP transcriptional reporter that is induced by heat shock in Arabidopsis. An automated fluorescence microscopy system which enables multiple roots to be imaged in rapid succession was used to quantitate Hsp17.6p:GFP response dynamics. Hsp17.6p:GFP signal increased with temperature increases from 28°C to 37°C. At 40°C the kinetics and localization of the response are markedly different from those at 37°C. This suggests that different mechanisms mediate heat shock responses above and below 37°C. Finally, we demonstrate that Hsp17.6p:GFP expression exhibits wave like dynamics in growing roots.ConclusionsThe RootScope system is a simple and powerful platform for investigating the heat shock response in plants.

【 授权许可】

Unknown   
© Kast et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.