An association of spleen volume and aortic diameter in patients and in mice with abdominal aortic aneurysm

BMC Surgery

Research Article

Fang-Da Li¹  Yue-Hong Zheng¹  Hao Nie¹  Rui Kang²  Xi-Ming Wang² 

[1] Department of Vascular Surgery, Peking Union Medical College Hospital, No.1 Shuaifuyuan, Dongdan, Dongcheng District, 100730, Beijing, China;Shandong Medical Imaging Research Institute, Shandong Provincial Key Laboratory of Diagnosis and Treatment of Cardio-Cerebral Vascular Diseases, Shandong University, NO, 324, Jingwu Road, 250021, Jinan, Shandong, China;

关键词: Abdominal aortic aneurysm;    Inflammation;    Ruptured;    Imaging;    Spleen volume;   

DOI  :  10.1186/s12893-017-0328-5

 received in 2017-06-21, accepted in 2017-11-27,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundTo investigate the potential mechanism of splenic enlargement in Ang II/APOE model and the associations between the spleen volume and the indices of abdominal aortic aneurysm (AAA) in human.MethodsTo investigate the changes of spleen volume on AAA formation, apolipoprotein E knockout (Apo E−/−) mice were treated with Ang II (1000 ng/kg/min) up to 28 days to generate AAA. We used Magnetic Resonance Imaging (MRI), liquid measurement, H&E and immunohistochemistry to analyze the morphological or pathological changes of spleen. To investigate the changes of spleen volume in human, a retrospective case-control study involving 30 male AAA patients and 25 male controls were performed. Spleen volume was measured on computed tomography images. Univariate analysis and multivariable sequential logistic regression analyses were used to analyze the association between spleen volume and maximal diameter (Dmax).ResultsIn Ang II/APOE model, we found splenic enlargement in mice with AAA compared with the sham group. Histopathological investigations revealed hypertrophies of splenic follicles and increased populations of CD3+ T cells. In clinic cohort study, univariate analysis revealed higher values in large AAA (Dmax > 5.5 cm,n = 15) compared with the small (Dmax < 5.5 cm,n = 15) for spleen volume (230.6 ± 64.5 cm3 vs. 170.0 ± 32.8 cm3; P = 0.0030). Regression analysis revealed a statistically significant positive linear correlation of spleen volume and Dmax of AAA (r = 0.3611;P = 0.0423).ConclusionsMimicking the splenic pathology observed in murine AAA model, there is a strong positive correlation between spleen volume and the Dmax in male AAA patients. As Dmax is a valuable predictor of AAA rupture, the spleen enlargement may be another indicator.

【 授权许可】

CC BY   
© The Author(s). 2017