In Vitro antibacterial and antibiotic-potentiation activities of four edible plants against multidrug-resistant gram-negative species

BMC Complementary and Alternative Medicine

Research Article

Dumitru Cojocaru¹  Marius Mihasan¹  Marius Stefan¹  Jean P Dzoyem²  Jules R Kuiate²  Victor Kuete²  Jaurès AK Noumedem³ 

[1] Department of Biochemistry and Molecular Biology, Faculty of Biology, University ALI Cuza, Iasi, Romania;Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon;Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon;Department of Biochemistry and Molecular Biology, Faculty of Biology, University ALI Cuza, Iasi, Romania;

关键词: Antibacterial activities;    Edible plants;    Gram-negative bacteria;    Multidrug resistance;    Efflux pumps;   

DOI  :  10.1186/1472-6882-13-190

 received in 2013-03-07, accepted in 2013-07-24,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe present study was designed to investigate the antibacterial activities of the methanol extracts of four Cameroonian edible plants, locally used to treat microbial infections, and their synergistic effects with antibiotics against a panel of twenty nine Gram-negative bacteria including Multi-drug resistant (MDR) phenotypes expressing active efflux pumps.MethodsThe broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) of the extracts [alone and in the presence of the efflux pumps inhibitor (EPI) Phenylalanine-Arginine β- Naphtylamide (PAβN)], and those of antibiotics in association with the two of the most active ones, Piper nigrum and Telfairia occidentalis. The preliminary phytochemical screening of the extracts was conducted according to the standard phytochemical methods.ResultsPhytochemical analysis showed the presence of alkaloids and flavonoids in all studied extracts. Other chemical classes of secondary metabolites were selectively present in the extracts. The results of the MIC determination indicated that the crude extracts from P. nigrum and V. amygdalina were able to inhibit the growth of all the twenty nine studied bacteria within a concentration range of 32 to 1024 μg/mL. At a similar concentration range (32 to 1024 μg/mL) the extract from T. occidentalis inhibited the growth of 93.1% of the tested microorganisms. At MIC/2 and MIC/5, synergistic effects were noted between the extracts from P. nigrum and T. occidentalis and seven of the tested antibiotics on more than 70% of the tested bacteria.ConclusionThe overall results of the present study provide information for the possible use of the studied edible plants extracts in the control of bacterial infections including MDR phenotypes.

【 授权许可】

Unknown   
© Noumedem et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.