Electroacupuncture improves cerebral blood flow and attenuates moderate ischemic injury via Angiotensin II its receptors-mediated mechanism in rats

BMC Complementary and Alternative Medicine

Research Article

Jing Li¹  Xuezhu Zhang¹  Yuanhao Du¹  Jingjun Cui²  Ying Ma³  Jiaojun He⁴ 

[1] Department of Acupuncture and Moxibustion Research Institute, First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China;Department of Neurology, Cangzhou Hospital of Integrated Traditional and Western Medicine of Hebei Province, 061001, Cangzhou, China;Editorial Department, Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China;Graduate School, Zhejiang Chinese Medical University, 310053, Hangzhou, China;

关键词: Cerebral Blood Flow;    Model Group;    Middle Cerebral Artery Occlusion;    Cerebral Blood Volume;    Time Phase;   

DOI  :  10.1186/1472-6882-14-441

 received in 2014-04-02, accepted in 2014-09-23,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundTo investigate the effects and potential mechanism of electroacupuncture intervention on expressions of Angiotensin II and its receptors-mediated signaling pathway in experimentally induced cerebral ischemia.MethodsTotally 126 male Wistar rats were randomly divided into control group, model group and EA group. The latter two were further divided into ten subgroups (n = 6) following Middle Cerebral Artery Occlusion (MCAO). Changes in regional cerebral blood flow (rCBF) and expressions of Angiotensin II and its receptors (AT1R, AT2R), as well as effector proteins in phosphatidyl inositol signal pathway were monitored before and at different times after MCAO.ResultsMCAO-induced decline of ipsilateral rCBF was partially suppressed by electroacupuncture, and contralateral blood flow was also superior to that of model group. Angiotensin II level was remarkably elevated immediately after MCAO, while electroacupuncture group exhibited significantly lower levels at 1 to 3 h and the value was significantly increased thereafter. The enhanced expression of AT1R was partially inhibited by electroacupuncture, while increased AT2R level was further induced. Electroacupuncture stimulation attenuated and postponed the upregulated-expressions of Gq and CaM these upregulations. ELISA results showed sharply increased expressions of DAG and IP3, which were remarkably neutralized by electroacupuncture.ConclusionsMCAO induced significant increases in expression of Angiotensin II and its receptor-mediated signal pathway. These enhanced expressions were significantly attenuated by electroacupuncture intervention, followed by reduced vasoconstriction and improved blood supply in ischemic region, and ultimately conferred beneficial effects on cerebral ischemia.

【 授权许可】

Unknown   
© Li et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.