Characterization of ex vivo cultured neuronal- and glial- like cells from human idiopathic epiretinal membranes

BMC Ophthalmology

Research Article

Morten C Moe¹  Andrea Facsk ó²  Sofija Andjelić³  Xhevat Lumi³  Marko Hawlina³  Goran Petrovski⁴  Jochen Graw⁵  Xiaohe Yan⁵ 

[1] Center for Eye Research, Department of Ophthalmology, Oslo University Hospital and University of Oslo, and Norwegian Center for Stem Cell Research, Kirkeveien 166, 0407, Oslo, Norway;Department of Ophthalmology, Faculty of Medicine, University of Szeged, Korányi fasor 10-11, 6720, Szeged, Hungary;Eye Hospital, University Medical Centre, Grablovičeva ulica 46, 1000, Ljubljana, Slovenia;Eye Hospital, University Medical Centre, Grablovičeva ulica 46, 1000, Ljubljana, Slovenia;Department of Ophthalmology, Faculty of Medicine, University of Szeged, Korányi fasor 10-11, 6720, Szeged, Hungary;Stem Cells and Eye Research Laboratory, Department of Biochemistry and Molecular Biology and Apoptosis and Genomics Research Group of the Hungarian Academy of Sciences, Faculty of Medicine, University of Debrecen, Egyetem tér 1., 4032, Debrecen, Hungary;Helmholtz Center Munich - German Research Center for Environmental Health, Institute of Developmental Genetics, Ingolstaedter Landstr. 1, D-85764, Neuherberg, Germany;

关键词: Idiopathic epiretinal membrane;    Glial- and neuronal cell growth;    Pluripotency;    Calcium dynamics;    Acetylcholine;    Mechanostimulation;    GFAP;    Nestin-1;    Sox2;    Pax2;   

DOI  :  10.1186/1471-2415-14-165

 received in 2014-10-11, accepted in 2014-12-11,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundCharacterization of the neuro-glial profile of cells growing out of human idiopathic epiretinal membranes (iERMs) and testing their proliferative and pluripotent properties ex vivo is needed to better understand the pathogenesis of their formation.MethodsiERMs obtained during uneventful vitrectomies were cultivated ex vivo under adherent conditions and assessed by standard morphological and immunocytochemical methods. The intracellular calcium dynamics of the outgrowing cells was assessed by fluorescent dye Fura-2 in response to acetylcholine (ACh)- or mechano- stimulation.ResultsThe cells from the iERMs formed sphere-like structures when cultured ex vivo. The diameter of the spheres increased by 5% at day 6 and kept an increasing tendency over a month time. The outgrowing cells from the iERM spheres had mainly glial- and some neuronal- like morphology. ACh- or mechano- stimulation of these cells induced intracellular calcium propagation in both cell types; in the neuronal-like cells resembling action potential from the soma to the dendrites. Immunocytochemistry confirmed presence of glial- and neuronal cell phenotype (GFAP and Nestin-1 positivity, respectively) in the iERMs, as well as presence of pluripotency marker (Sox2).ConclusioniERMs contain cells of neuronal- and glial- like origin which have proliferative and pluripotent potential, show functionality reflected through calcium dynamics upon ACh and mechano- stimulation, and a corresponding molecular phenotype.

【 授权许可】

Unknown   
© Andjelić et al.; licensee BioMed Central. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.