Comparative genomics and transcriptomics of lineages I, II, and III strains of Listeria monocytogenes

BMC Genomics

Research Article

Alfred Pühler¹  Alexander Goesmann¹  Sebastian Oehm¹  Sonja Vorwerk²  Uwe Kärst³  Carmen Buchrieser⁴  Christophe Rusniok⁴  Patricia Garrido⁴  Philippe Glaser⁵  Rainer Merkl⁶  Mobarak Abu Mraheil⁷  Walid Mohamed⁷  Benjamin Izar⁷  Silke Schaffrath⁷  André Billion⁷  Christiane Steinweg⁷  Carsten Tobias Kuenne⁷  Torsten Hain⁷  Rohit Ghai⁷  Trinad Chakraborty⁷  Eugen Domann⁷  Werner Goebel⁸ 

[1] Center for Biotechnology, University of Bielefeld, D-33594, Bielefeld, Germany;Febit Biomed GmbH, Im Neuenheimer Feld 519, D-69120, Heidelberg, Germany;Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstraße 7, 38124, Braunschweig, Germany;Institut Pasteur, Biologie des Bactéries Intracellulaires and CNRS URA 2171, 75724, Paris, France;Institut Pasteur, Laboratoire Evolution et Génomique Bactériennes and CNRS URA 2171, 75724, Paris, France;Institut für Biophysik und physikalische Biochemie, Universität Regensburg, Universitätstrasse 31, D-93053, Regensburg, Germany;Institute of Medical Microbiology, Justus-Liebig-University, Schubertstrasse 81, D-35392, Giessen, Germany;Max von Pettenkofer-Institut for Hygiene and Medical Microbiology, Ludwig Maximilians-University München, Pettenkoferstrasse 9a, D-80336, Munich, Germany;

关键词: Listeria monocytogenes;    Lineage;    Comparative genomics;    Gene decay;    Comparative transcriptomics;    Flagella;    Prophage;    Monocin;    Isogenic deletion mutants;    Murine infection;   

DOI  :  10.1186/1471-2164-13-144

 received in 2011-08-25, accepted in 2012-04-24,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundListeria monocytogenes is a food-borne pathogen that causes infections with a high-mortality rate and has served as an invaluable model for intracellular parasitism. Here, we report complete genome sequences for two L. monocytogenes strains belonging to serotype 4a (L99) and 4b (CLIP80459), and transcriptomes of representative strains from lineages I, II, and III, thereby permitting in-depth comparison of genome- and transcriptome -based data from three lineages of L. monocytogenes. Lineage III, represented by the 4a L99 genome is known to contain strains less virulent for humans.ResultsThe genome analysis of the weakly pathogenic L99 serotype 4a provides extensive evidence of virulence gene decay, including loss of several important surface proteins. The 4b CLIP80459 genome, unlike the previously sequenced 4b F2365 genome harbours an intact inlB invasion gene. These lineage I strains are characterized by the lack of prophage genes, as they share only a single prophage locus with other L. monocytogenes genomes 1/2a EGD-e and 4a L99. Comparative transcriptome analysis during intracellular growth uncovered adaptive expression level differences in lineages I, II and III of Listeria, notable amongst which was a strong intracellular induction of flagellar genes in strain 4a L99 compared to the other lineages. Furthermore, extensive differences between strains are manifest at levels of metabolic flux control and phosphorylated sugar uptake. Intriguingly, prophage gene expression was found to be a hallmark of intracellular gene expression. Deletion mutants in the single shared prophage locus of lineage II strain EGD-e 1/2a, the lma operon, revealed severe attenuation of virulence in a murine infection model.ConclusionComparative genomics and transcriptome analysis of L. monocytogenes strains from three lineages implicate prophage genes in intracellular adaptation and indicate that gene loss and decay may have led to the emergence of attenuated lineages.

【 授权许可】

Unknown   
© Hain et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.