Ifosfamide-loaded poly (lactic-co-glycolic acid) PLGA-dextran polymeric nanoparticles to improve the antitumor efficacy in Osteosarcoma

BMC Cancer

Research Article

Jie-Zuan Yang¹  Li-Feng Wang²  Yi-Jun Zhang²  Xiang-Jin Lin²  Bin Chen² 

[1] Department of Laboratoire Central, The First Affiliated Hospital of Medical School of Zhejiang University, 310003, Hangzhou, China;Department of Orthopedic, The First Affiliated Hospital of Medical School of Zhejiang University, No. 79 Qingchun Road, 310003, Hangzhou, Zhejiang, China;

关键词: Ifosfamide;    Osteosarcoma;    Polymeric nanoparticles;    Block copolymer;    Apoptosis;   

DOI  :  10.1186/s12885-015-1735-6

 received in 2015-04-07, accepted in 2015-10-08,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundOsteosarcoma is a typical bone cancer that primarily affects adolescents. The therapeutic activity of drugs is limited by their severe drug-related toxicities, therefore, a therapeutic approach which is less toxic and highly effective in tumor is of utmost importance.MethodIn this study, ifosfamide-loaded poly (lactic-co-glycolic acid) (PLGA)-dextran polymeric nanoparticles (PD/IFS) was developed and studied its anticancer efficacy against multiple osteosarcoma cancer cells. The drug-loaded nanoparticle was characterized for physical and biological characterizations.ResultsThe formulated PD/IFS showed a high drug loading capacity and displayed a pH-sensitive release pattern, with a sustained release profile of the IFS. PD/IFS nanoparticles exhibited remarkable in vitro anticancer activity comparable to that of free IFS solution in a concentration dependent manner in MG63 and Saos-2 cancer cells. PLGA-dextran by itself did not affect cell viability of cancer cells indicating its excellent biocompatibility. The formulation exhibited significantly higher PARP and caspase-3/7 expression in both the cancer cells.ConclusionOur study successfully demonstrated that nanoparticulate encapsulation of antitumor agent will increase the therapeutic efficacy and exhibit a greater induction of apoptosis and cell death.

【 授权许可】

CC BY   
© Chen et al. 2015