Rhaphidophora korthalsii modulates peripheral blood natural killer cell proliferation, cytokine secretion and cytotoxicity

BMC Complementary and Alternative Medicine

Research Article

Boon Kee Beh¹  Noorjahan Banu Alitheen²  Wan Yong Ho²  Abdul Manaf Ali³  Swee Keong Yeap⁴  Abdul Rahman Omar⁵ 

[1] Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400, Serdang, Selangor, Malaysia;Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400, Serdang, Selangor, Malaysia;Faculty of Agriculture and Biotechnology, University Sultan Zainal Abidin, 20400, Kampus Kota, Jalan Sultan Mahmud, Kuala Terengganu, Malaysia;Institute of Bioscience, University Putra Malaysia, Serdang, Selangor, Malaysia;Institute of Bioscience, University Putra Malaysia, Serdang, Selangor, Malaysia;Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University Putra Malaysia, Serdang, Selangor, Malaysia;

关键词: Rhaphidophora korthalsii;    NK cell;    Immunomodulation;   

DOI  :  10.1186/1472-6882-13-145

 received in 2012-08-26, accepted in 2013-06-10,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundRhaphidophora korthalsii (Araceae) is a root-climber plant which has been widely used in Chinese traditional medicine for cancer and skin disease treatment. Previous reports have recorded its immunomodulatory effects on mice splenocyte and human peripheral blood. This study investigated the potential immunostimulatory effect of Rhaphidophora korthalsii on human PBMC enriched NK cell.MethodsPBMC was exposed to various concentrations of R. korthalsii extract and the T and NK cell population in the control and extract treated PBMC were identified by immunophenotyping. Intracellular perforin and granzyme B expressions were detected by flow cytometry and extra-cellular Granzyme B, IFN-γ and TNF-α production in the isolated NK cells were determined by ELISA. The cytotoxicity of effector NK cell towards target K562 cell was assessed by CytoTox 96 assay.ResultsRhaphidophora korthalsii methanol extract significantly increased PBMC NK cell population and intracellular perforin and granzyme B expressions. Moreover, the extract also enhanced the secretion of IFN-γ and TNF-α which subsequently enhanced the cytotoxicity of NK cell against the NK sensitive target K562 cell line. NK cell enriched with extract treated PBMC showed better activation than NK cell directly treated with the extract.ConclusionOur findings indicated a potential IL-2 free immunotherapy through direct and indirect R. korthalsii stimulation on NK cell activation.

【 授权许可】

Unknown   
© Yeap et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.