Metabolomic analysis of pathways related to rice grain chalkiness by a notched-belly mutant with high occurrence of white-belly grains

BMC Plant Biology

Research Article

Ganghua Li¹  Xincheng Zhang¹  Yutong Jiang¹  Shaohua Wang¹  Zunxin Wang¹  Yanfeng Ding¹  Zhaomiao Lin²  Zhenghui Liu³  Danny Alexander⁴ 

[1] College of Agronomy, Nanjing Agricultural University/Key Laboratory of Crop Physiology Ecology and Production Management, Ministry of Agriculture, 210095, Nanjing, People’s Republic of China;College of Agronomy, Nanjing Agricultural University/Key Laboratory of Crop Physiology Ecology and Production Management, Ministry of Agriculture, 210095, Nanjing, People’s Republic of China;Crops Research Institute, Fujian Academy of Agricultural Sciences, 350013, Fuzhou, People’s Republic of China;College of Agronomy, Nanjing Agricultural University/Key Laboratory of Crop Physiology Ecology and Production Management, Ministry of Agriculture, 210095, Nanjing, People’s Republic of China;Jiangsu Collaborative Innovation Center for Modern Crop Production, 210095, Nanjing, People’s Republic of China;Metabolon Inc, 27713, Durham, NC, USA;

关键词: Grain chalkiness;    Metabolomic analysis;    Notched-belly mutants;    Rice;    White-belly;   

DOI  :  10.1186/s12870-017-0985-7

 received in 2016-08-24, accepted in 2017-01-25,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundGrain chalkiness is a highly undesirable trait deleterious to rice appearance and milling quality. The physiological and molecular foundation of chalkiness formation is still partially understood, because of the complex interactions between multiple genes and growing environments.ResultsWe report the untargeted metabolomic analysis of grains from a notched-belly mutant (DY1102) with high percentage of white-belly, which predominantly occurs in the bottom part proximal to the embryo. Metabolites in developing grains were profiled on the composite platforms of UPLC/MS/MS and GC/MS. Sampling times were 5, 10, 15, and 20 days after anthesis, the critical time points for chalkiness formation. A total of 214 metabolites were identified, covering most of the central metabolic pathways and partial secondary pathways including amino acids, carbohydrates, lipids, cofactors, peptides, nucleotides, phytohormones, and secondary metabolites. A comparison of the bottom chalky part and the upper translucent part of developing grains of DY1102 resulted in 180 metabolites related to chalkiness formation.ConclusionsGenerally, in comparison to the translucent upper part, the chalky endosperm had lower levels of metabolites regarding carbon and nitrogen metabolism for synthesis of storage starch and protein, which was accompanied by perturbation of pathways participating in scavenging of reactive oxygen species, osmorugulation, cell wall synthesis, and mineral ion homeostasis. Based on these results, metabolic mechanism of chalkiness formation is discussed, with the role of embryo highlighted.

【 授权许可】

CC BY   
© The Author(s). 2017