Anti-angiotensin converting enzyme (ACE) proteins from mycelia of Ganoderma lucidum (Curtis) P. Karst

BMC Complementary and Alternative Medicine

Research Article

Noorlidah Abdullah¹  Nurhuda Mohamad Ansor²  Norhaniza Aminudin³ 

[1] Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia;Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia;Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bandar Putra Bertam, 13200, Kepala Batas, Penang, Malaysia;Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia;University of Malaya Centre for Proteomics Research, University of Malaya, 50603, Kuala Lumpur, Malaysia;

关键词: ACE inhibitory proteins;    Bioactive peptides/proteins;    Lingzhi;    Hypertension;    MALDI-TOF/TOF MS;   

DOI  :  10.1186/1472-6882-13-256

 received in 2013-05-21, accepted in 2013-09-17,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundGanoderma lucidum has been purported as a potent remedy in the treatment and prevention of several ailments, including hypertension. This study aimed to explore the anti-ACE potential of protein fractions from the mycelia of G. lucidum.MethodsGanoderma lucidum mycelia were cultivated by submerged fermentation in a liquid medium containing brown sugar and spent brewer’s yeast. Intracellular proteins were fractionated from mycelia crude water extract by ammonium sulphate precipitation, and their angiotensin converting enzyme inhibitory activity was evaluated. The potential anti-ACE protein fractions were further separated by RP-HPLC and characterised using proteomics platforms.ResultsPreliminary result demonstrated that the mycelia crude water extract inhibited ACE at IC50 value of 1.134 ± 0.036 mg/mL. Following protein fractionation and HPLC purification, the presence of highly potential anti-ACE proteins with the IC50 values less than 200 μg/mL was detected. Characterisation of these proteins demonstrated the presence of four different antihypertensive-related proteins involved in the regulation of blood pressure through different mechanisms.ConclusionsThis study suggests that the mycelia of G. lucidum has high potential in lowering blood pressure level due to the presence of several antihypertensive-related proteins such as cystathionine beta synthase-like protein, DEAD/DEAH box helicase-like protein, paxillin-like protein, and alpha/beta hydrolase-like protein.

【 授权许可】

Unknown   
© Mohamad Ansor et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.