Molecular foundations of chilling-tolerance of modern maize

BMC Genomics

Research Article

Marek Skoneczny¹  Joanna Trzcinska-Danielewicz²  Iwona Kuciara²  Jan Fronk²  Katarzyna Hetmańczyk³  Paweł Sowiński⁴  Marcin Grzybowski⁴  Jarosław Szczepanik⁴  Danuta Solecka⁴  Alicja Sobkowiak⁴  Maciej Jończyk⁴  Józef Adamczyk⁵ 

[1] Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5A, 02-106, Warszawa, Poland;Department of Molecular Biology, Institute of Biochemistry, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warszawa, Poland;Department of Molecular Biology, Institute of Biochemistry, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warszawa, Poland;Present address: Department of Genetics, Institute of Psychiatry and Neurology in Warsaw, Sobieskiego 9, 02-957, Warsaw, Poland;Department of Plant Molecular Ecophysiology, Institute of Plant Experimental Biology and Biotechnology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warszawa, Poland;Plant Breeding Smolice Co. Ltd., Smolice 146, 63-740, Kobylin, Poland;

关键词: Acclimatization;    Cell walls;    Cold-sensitivity;    Maize;    Microarrays;    miRNA;    Photosynthesis;    Seedling;   

DOI  :  10.1186/s12864-016-2453-4

 received in 2015-04-27, accepted in 2016-02-09,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundRecent progress in selective breeding of maize (Zea mays L.) towards adaptation to temperate climate has allowed the production of inbred lines withstanding cold springs with temperatures below 8 °C or even close to 0 °C, indicating that despite its tropical origins maize is not inherently cold-sensitive.ResultsHere we studied the acclimatory response of three maize inbred lines of contrasting cold-sensitivity selected basing on multi-year routine field data. The field observations were confirmed in the growth chamber. Under controlled conditions the damage to the photosynthetic apparatus due to severe cold treatment was the least in the cold-tolerant line provided that it had been subjected to prior moderate chilling, i.e., acclimation. The cold-sensitive lines performed equally poorly with or without acclimation. To uncover the molecular basis of the attained cold-acclimatability we performed comparative transcriptome profiling of the response of the lines to the cold during acclimation phase by means of microarrays with a statistical and bioinformatic data analysis.ConclusionsThe analyses indicated three mechanisms likely responsible for the cold-tolerance: acclimation-dependent modification of the photosynthetic apparatus, cell wall properties, and developmental processes. Those conclusions supported the observed acclimation of photosynthesis to severe cold at moderate chilling and were further confirmed by experimentally showing specific modification of cell wall properties and repression of selected miRNA species, general regulators of development, in the cold-tolerant line subjected to cold stress.

【 授权许可】

CC BY   
© Sobkowiak et al. 2016