dConsensus: a tool for displaying domain assignments by multiple structure-based algorithms and for construction of a consensus assignment

BMC Bioinformatics

Software

Stella Veretnik¹  Philip E Bourne²  Kieran Alden³ 

[1] San Diego Supercomputer Center, University of California San Diego, 9500 Gilman Dr., 92093-0743, La Jolla, CA, USA;Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, 9500 Gilman Dr., 92093-0636, La Jolla, CA, USA;York Centre for Complex Systems Analysis (YCCSA), University of York, YO10 5DD, Heslington, York, UK;

关键词: Protein Data Bank;    Protein Chain;    Secondary Structure Element;    Reliability Score;    Algorithmic Method;   

DOI  :  10.1186/1471-2105-11-310

 received in 2010-01-14, accepted in 2010-06-09,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundPartitioning of a protein into structural components, known as domains, is an important initial step in protein classification and for functional and evolutionary studies. While the systematic assignments of domains by human experts exist (CATH and SCOP), the introduction of high throughput technologies for structure determination threatens to overwhelm expert approaches. A variety of algorithmic methods have been developed to expedite this process, allowing almost instant structural decomposition into domains. The performance of algorithmic methods can approach 85% agreement on the number of domains with the consensus reached by experts. However, each algorithm takes a somewhat different conceptual approach, each with unique strengths and weaknesses. Currently there is no simple way to automatically compare assignments from different structure-based domain assignment methods, thereby providing a comprehensive understanding of possible structure partitioning as well as providing some insight into the tendencies of particular algorithms. Most importantly, a consensus assignment drawn from multiple assignment methods can provide a singular and presumably more accurate view.ResultsWe introduce dConsensus http://pdomains.sdsc.edu/dConsensus; a web resource that displays the results of calculations from multiple algorithmic methods and generates a domain assignment consensus with an associated reliability score. Domain assignments from seven structure-based algorithms - PDP, PUU, DomainParser2, NCBI method, DHcL, DDomains and Dodis are available for analysis and comparison alongside assignments made by expert methods. The assignments are available for all protein chains in the Protein Data Bank (PDB). A consensus domain assignment is built by either allowing each algorithm to contribute equally (simple approach) or by weighting the contribution of each method by its prior performance and observed tendencies. An analysis of secondary structure around domain and fragment boundaries is also available for display and further analysis.ConclusiondConsensus provides a comprehensive assignment of protein domains. For the first time, seven algorithmic methods are brought together with no need to access each method separately via a webserver or local copy of the software. This aggregation permits a consensus domain assignment to be computed. Comparison viewing of the consensus and choice methods provides the user with insights into the fundamental units of protein structure so important to the study of evolutionary and functional relationships.

【 授权许可】

Unknown   
© Alden et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.