Anti-apoptotic effect of claudin-1 in tamoxifen-treated human breast cancer MCF-7 cells

BMC Cancer

Research Article

Satoko Morohashi¹  Harue Akasaka¹  Jun Kondo¹  Hiroshi Kijima¹  Fuyuki Sato¹  Yunyan Wu²  Yang Liu²  Hiroki Odagiri³  Kenichi Hakamada³ 

[1] Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine, 036-8562, Hirosaki, Japan;Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine, 036-8562, Hirosaki, Japan;Department of Pathology, College of Basic Medical Sciences, China Medical University, 110001, Shenyang, China;Department of Surgery, Hirosaki University Graduate School of Medicine, 036-8562, Hirosaki, Japan;

关键词: Tamoxifen;    Subcellular Localization;    Tamoxifen Treatment;    Nuclear Condensation;    Immunoreaction Enhancer Solution;   

DOI  :  10.1186/1471-2407-10-548

 received in 2010-05-08, accepted in 2010-10-12,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundClaudin-1 is a membrane protein of tight junctions, and is associated with the development of various cancers. However, the significance of claudin-1 expression in cancer cells is not well understood. Here, we showed for the first time the anti-apoptotic effect of claudin-1 in human breast cancer MCF-7 cells.MethodsHuman breast cancer MCF-7 and T47 D cells were treated with or without tamoxifen, siRNA against claudin-1, or tamoxifen and claudin-1 siRNA. The samples were analyzed by RT-PCR, Western blotting or immunofluorescent staining.ResultsThe expression of claudin-1 was upregulated in tamoxifen-treated MCF-7 cells, whereas the expression of claudin-1 was not altered in tamoxifen-treated T47 D cells. Knockdown of claudin-1 by siRNA increased the amount of poly (ADP-ribose) polymerase (PARP) regardless of tamoxifen treatment in MCF-7 cells, but not T47 D cells. In the cell membranes of the MCF-7 cells, tamoxifen treatment increased the amount of claudin-1, but decreased the amount of β-catenin. Claudin-1 siRNA increased the amount of E-cadherin in the cytoplasm of the MCF-7 cells as well as the amount of β-catenin in their cell membranes.ConclusionThese results indicate that claudin-1 has anti-apoptotic effects, and is involved in the regulation of the expression and subcellular localization of β-catenin and E-cadherin in MCF-7, but not T47 D cells.

【 授权许可】

Unknown   
© Akasaka et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.