Adaptation of maize source leaf metabolism to stress related disturbances in carbon, nitrogen and phosphorus balance

BMC Genomics

Research Article

Nina Zellerhoff¹  Marcel Bucher¹  Uwe Sonnewald²  Urte Schlüter²  Uwe Scholz³  Christian Colmsee³  Holger Fahnenstich⁴  Andreas PM Weber⁵  Andrea Bräutigam⁵ 

[1] Botanical Institute, Albertus Magnus Platz, University of Cologne, 90923, Cologne, Germany;Department of Biology, Division of Biochemistry, Friedrich-Alexander-University Erlangen-Nuremberg, Staudtstr. 5, 91058, Erlangen, Germany;Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Bioinformatics and Information Technology Group, Corrensstr. 3, 06466, Gatersleben, Germany;Metanomics GmbH, Tegeler Weg 33, 10589, Berlin, Germany;Plant Biochemistry Department, Heinrich Heine University Düsseldorf, 40225, Duesseldorf, Germany;

关键词: Abiotic stress;    Nutrient stress;    Transcriptome;    Metabolome;    Ionome;    Maize;   

DOI  :  10.1186/1471-2164-14-442

 received in 2013-03-06, accepted in 2013-06-21,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundAbiotic stress causes disturbances in the cellular homeostasis. Re-adjustment of balance in carbon, nitrogen and phosphorus metabolism therefore plays a central role in stress adaptation. However, it is currently unknown which parts of the primary cell metabolism follow common patterns under different stress conditions and which represent specific responses.ResultsTo address these questions, changes in transcriptome, metabolome and ionome were analyzed in maize source leaves from plants suffering low temperature, low nitrogen (N) and low phosphorus (P) stress. The selection of maize as study object provided data directly from an important crop species and the so far underexplored C4 metabolism. Growth retardation was comparable under all tested stress conditions. The only primary metabolic pathway responding similar to all stresses was nitrate assimilation, which was down-regulated. The largest group of commonly regulated transcripts followed the expression pattern: down under low temperature and low N, but up under low P. Several members of this transcript cluster could be connected to P metabolism and correlated negatively to different phosphate concentration in the leaf tissue. Accumulation of starch under low temperature and low N stress, but decrease in starch levels under low P conditions indicated that only low P treated leaves suffered carbon starvation.ConclusionsMaize employs very different strategies to manage N and P metabolism under stress. While nitrate assimilation was regulated depending on demand by growth processes, phosphate concentrations changed depending on availability, thus building up reserves under excess conditions. Carbon and energy metabolism of the C4 maize leaves were particularly sensitive to P starvation.

【 授权许可】

Unknown   
© Schlüter et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.