Genetics of Na+ exclusion and salinity tolerance in Afghani durum wheat landraces

BMC Plant Biology

Research Article

Nawar Jalal Shamaya¹  Yuri Shavrukov²  Stuart John Roy²  Peter Langridge²  Mark Tester³ 

[1] Department of Field Crops, College of Agriculture, University of Baghdad, Al-Jadriyah, Baghdad, Iraq;School of Agriculture, Food and Wine, The University of Adelaide, 5064, Urrbrae, SA, Australia;Australian Centre for Plant Functional Genomics, The University of Adelaide, 5064, Urrbrae, SA, Australia;School of Agriculture, Food and Wine, The University of Adelaide, 5064, Urrbrae, SA, Australia;Australian Centre for Plant Functional Genomics, The University of Adelaide, 5064, Urrbrae, SA, Australia;School of Agriculture, Food and Wine, The University of Adelaide, 5064, Urrbrae, SA, Australia;Australian Centre for Plant Functional Genomics, The University of Adelaide, 5064, Urrbrae, SA, Australia;Center for Desert Agriculture, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, 4700, Thuwal, Kingdom of Saudi Arabia;

关键词: Salinity tolerance;    Leaf Na concentration;    K/Na ratio;    Bulk Segregant analysis;    SNP markers;    HKT1;    5;   

DOI  :  10.1186/s12870-017-1164-6

 received in 2017-04-21, accepted in 2017-11-08,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundSelecting for low concentration of Na+ in the shoot provides one approach for tackling salinity stress that adversely affects crop production. Novel alleles for Na+ exclusion can be identified and then introduced into elite crop cultivars.ResultsWe have identified loci associated with lower Na+ concentration in leaves of durum wheat landraces originating from Afghanistan. Seedlings of two F2 populations derived from crossings between Australian durum wheat (Jandaroi) and two Afghani landraces (AUS-14740 and AUS-14752) were grown hydroponically and evaluated for Na+ and K+ concentration in the third leaf. High heritability was found for both third leaf Na+ concentration and the K+/Na+ ratio in both populations. Further work focussed on line AUS-14740. Bulk segregant analysis using 9 K SNP markers identified two loci significantly associated with third leaf Na+ concentration. Marker regression analysis showed a strong association between all traits studied and a favourable allele originating from AUS-14740 located on the long arm of chromosome 4B.ConclusionsThe candidate gene in the relevant region of chromosome 4B is likely to be the high affinity K+ transporter B1 (HKT1;5-B1). A second locus associated with third leaf Na+ concentration was located on chromosome 3BL, with the favourable allele originating from Jandaroi; however, no candidate gene can be identified.

【 授权许可】

CC BY   
© The Author(s). 2017