Anticancer activity of 7-epiclusianone, a benzophenone from Garcinia brasiliensis, in glioblastoma

BMC Complementary and Alternative Medicine

Research Article

María Sol Brassesco¹  Luiz Gonzaga Tone²  Kleiton Silva Borges²  Carlos Alberto Scrideli²  Marisa Ionta³  Marcelo Henrique dos Santos⁴  Leilane Sales⁵  Jaqueline Carvalho de Oliveira⁵  Julia Alejandra Pezuk⁶ 

[1] Department of Biology, Faculty of Philosophy, Sciences and Letters at Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, SP, Brazil;Department of Paediatrics, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, SP, Brazil;Institute of Biomedical Sciences, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 700, 37130-000, Alfenas, MG, Brazil;Institute of Chemistry, Federal University of Viçosa, Avenida Peter Henry Rolfs, s/n, 36570-900, Viçosa, MG, Brazil;Institute of Natural Sciences, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 700, 37130-000, Alfenas, MG, Brazil;Molecular Oncology Center, Sirio Libanes Hospital, Rua Dona Adma Jafet, 115, 01308-050, Sao Paulo, SP, Brazil;

关键词: Glioblastoma;    epiclusianone;    Viability;    Apoptosis;    Cell cycle;   

DOI  :  10.1186/s12906-015-0911-1

 received in 2015-07-23, accepted in 2015-10-12,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundGlioblastoma is the most common tumor of the central nervous system and one of the hardest tumors to treat. Consequently, the search for novel therapeutic options is imperative. 7-epiclusianone, a tetraprenylated benzophenone isolated from the epicarp of the native plant Garcinia brasiliensis, exhibits a range of biological activities but its prospect anticancer activity is underexplored. Thus, the aim of the present study was to evaluate the influence of 7-epiclusianone on proliferation, clonogenic capacity, cell cycle progression and induction of apoptosis in two glioblastoma cell lines (U251MG and U138MG).MethodsCell viability was measured by the MTS assay; for the clonogenic assay, colonies were stained with Giemsa and counted by direct visual inspection; For cell cycle analysis, cells were stained with propidium iodide and analyzed by cytometry; Cyclin A expression was determined by immunoblotting; Apoptotic cell death was determined by annexin V fluorescein isothiocyanate labeling and Caspase-3 activity in living cells.ResultsViability of both cell lines was drastically inhibited; moreover, the colony formation capacity was significantly reduced, demonstrating long-term effects even after removal of the drug. 7-epiclusianone treatment at low concentrations also altered cell cycle progression, decreased the S and G2/M populations and at higher concentrations increased the number of cells at sub-G1, in concordance with the increase of apoptotic cells.ConclusionThe present study demonstrates for the first time the anticancer potential of 7-epiclusianone against glioblastoma cells, thus meriting its further investigation as a potential therapeutic agent.

【 授权许可】

CC BY   
© Sales et al. 2015