Transcriptome analysis reveals absence of unintended effects in drought-tolerant transgenic plants overexpressing the transcription factor ABF3

BMC Genomics

Research Article

Brian Miki¹  Ashraf Abdeen²  Jaimie Schnell³ 

[1] Agriculture and Agri-Food Canada, 960 Carling Ave., K1A 0C6, Ottawa, ON, Canada;Agriculture and Agri-Food Canada, 960 Carling Ave., K1A 0C6, Ottawa, ON, Canada;Department of Biology, McGill University, 1205 Docteur Penfield Ave., H3A 1B1, Montreal, QC, Canada;Agriculture and Agri-Food Canada, 960 Carling Ave., K1A 0C6, Ottawa, ON, Canada;Plant and Biotechnology Risk Assessment Unit, Canadian Food Inspection Agency, 1400 Merivale Rd., K1A 0Y9, Ottawa, ON, Canada;

关键词: Drought Stress;    Unintended Effect;    Plant Line;    Late Embryogenesis Abundant;    Drought Response;   

DOI  :  10.1186/1471-2164-11-69

 received in 2009-08-12, accepted in 2010-01-28,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundPlants engineered for abiotic stress tolerance may soon be commercialized. The engineering of these plants typically involves the manipulation of complex multigene networks and may therefore have a greater potential to introduce pleiotropic effects than the simple monogenic traits that currently dominate the plant biotechnology market. While research on unintended effects in transgenic plant systems has been instrumental in demonstrating the substantial equivalence of many transgenic plant systems, it is essential that such analyses be extended to transgenic plants engineered for stress tolerance. Drought-tolerant Arabidopsis thaliana were engineered through overexpression of the transcription factor ABF3 in order to investigate unintended pleiotropic effects. In order to eliminate position effects, the Cre/lox recombination system was used to create control plant lines that contain identical T-DNA insertion sites but with the ABF3 transgene excised. This additionally allowed us to determine if Cre recombinase can cause unintended effects that impact the transcriptome.ResultsMicroarray analysis of control plant lines that underwent Cre-mediated excision of the ABF3 transgene revealed only two genes that were differentially expressed in more than one plant line, suggesting that the impact of Cre recombinase on the transcriptome was minimal. In the absence of drought stress, overexpression of ABF3 had no effect on the transcriptome, but following drought stress, differences were observed in the gene expression patterns of plants overexpressing ABF3 relative to control plants. Examination of the functional distribution of the differentially expressed genes revealed strong similarity indicating that unintended pathways were not activated.ConclusionsThe action of ABF3 is tightly controlled in Arabidopsis. In the absence of drought stress, ectopic activation of drought response pathways does not occur. In response to drought stress, overexpression of ABF3 results in a reprogramming of the drought response, which is characterized by changes in the timing or strength of expression of some drought response genes, without activating any unexpected gene networks. These results illustrate that important gene networks are highly regulated in Arabidopsis and that engineering stress tolerance may not necessarily cause extensive changes to the transcriptome.

【 授权许可】

Unknown   
© Abdeen et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.