Soil water stress affects both cuticular wax content and cuticle-related gene expression in young saplings of maritime pine (Pinus pinasterAit)

BMC Plant Biology

Research Article

Frédéric Danjon¹  Antoine Grosbois¹  Céline Meredieu¹  Didier Bert¹  Christophe Plomion¹  Grégoire Le Provost¹  Céline Lalanne¹  Jean-Marc Gion²  Patricio Ramos Campos³  Frédéric Domergue⁴ 

[1] INRA, UMR 1202, BIOGECO, F-33610, Cestas, France;Univ. Bordeaux, BIOGECO, UMR 1202, F-33400, Talence, France;INRA, UMR 1202, BIOGECO, F-33610, Cestas, France;Univ. Bordeaux, BIOGECO, UMR 1202, F-33400, Talence, France;CIRAD, UMR AGAP, Campus de Baillarguet TA 10C, F-34398, Montpellier Cedex 5, France;Instituto Biología Vegetal y Biotecnología, Universidad de Talca, 2 Norte 685, Talca, Chile;Univ. Bordeaux, Laboratoire de Biogenèse Membranaire, UMR5200, F-33000, Bordeaux, France;CNRS, Laboratoire de Biogenèse Membranaire, UMR5200, F-33000, Bordeaux, France;

关键词: Cuticle biosynthesis;    Drought;    Edaphic stress;    Field experiment;    Gene expression;    Maritime pine;   

DOI  :  10.1186/1471-2229-13-95

 received in 2012-12-14, accepted in 2013-06-28,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe cuticle is a hydrophobic barrier located at the aerial surface of all terrestrial plants. Recent studies performed on model plants, such as Arabidopsis thaliana, have suggested that the cuticle may be involved in drought stress adaptation, preventing non-stomatal water loss. Although forest trees will face more intense drought stresses (in duration and intensity) with global warming, very few studies on the role of the cuticle in drought stress adaptation in these long-lived organisms have been so far reported.ResultsThis aspect was investigated in a conifer, maritime pine (Pinus pinaster Ait.), in a factorial design with two genetic units (two half-sib families with different growth rates) and two treatments (irrigated vs non-irrigated), in field conditions. Saplings were grown in an open-sided greenhouse and half were irrigated three times per week for two growing seasons. Needles were sampled three times per year for cuticular wax (composition and content) and transcriptome (of 11 genes involved in cuticle biosynthesis) analysis. Non-irrigated saplings (i) had a higher cuticular wax content than irrigated saplings and (ii) overexpressed most of the genes studied. Both these trends were more marked in the faster growing family.ConclusionsThe higher cuticular wax content observed in the non-irrigated treatment associated with strong modifications in products from the decarbonylation pathway suggest that cuticular wax may be involved in drought stress adaptation in maritime pine. This study provides also a set of promising candidate genes for future forward genetic studies in conifers.

【 授权许可】

CC BY   
© Le Provost et al.; licensee BioMed Central Ltd. 2013