Proteomic analysis of free-living Bradyrhizobium diazoefficiens: highlighting potential determinants of a successful symbiosis

BMC Genomics

Research Article

Lygia Vitoria Galli-Terasawa¹  Luciano Paulino da Silva²  Carlos Bloch²  Amanda Alves Paiva Rolla³  Mariangela Hungria³  Jesiane Stefânia da Silva Batista⁴  Douglas Fabiano Gomes⁵ 

[1] Departamento de Genética, Universidade Federal do Paraná, C.P. 19031, 81531-900, Curitiba, PR, Brazil;Embrapa Recursos Genéticos e Biotecnologia, PqEB s/n°, 70770-901, Brasília, DF, Brazil;Embrapa Soja, Embrapa Soja, C.P. 231, 86001-970, Londrina, Paraná, Brazil;Embrapa Soja, Embrapa Soja, C.P. 231, 86001-970, Londrina, Paraná, Brazil;Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, C.P. 6001, 84030-900, Ponta Grossa, PR, Brazil;Embrapa Soja, Embrapa Soja, C.P. 231, 86001-970, Londrina, Paraná, Brazil;Departamento de Genética, Universidade Federal do Paraná, C.P. 19031, 81531-900, Curitiba, PR, Brazil;

关键词: Symbiosis;    Nitrogen fixation;    Two-dimensional proteomics;    RT-qPCR;    Bradyrhizobium;   

DOI  :  10.1186/1471-2164-15-643

 received in 2014-05-05, accepted in 2014-07-25,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundStrain CPAC 7 (=SEMIA 5080) was recently reclassified into the new species Bradyrhizobium diazoefficiens; due to its outstanding efficiency in fixing nitrogen, it has been used in commercial inoculants for application to crops of soybean [Glycine max (L.) Merr.] in Brazil and other South American countries. Although the efficiency of B. diazoefficiens inoculant strains is well recognized, few data on their protein expression are available.ResultsWe provided a two-dimensional proteomic reference map of CPAC 7 obtained under free-living conditions, with the successful identification of 115 spots, representing 95 different proteins. The results highlighted the expression of molecular determinants potentially related to symbiosis establishment (e.g. inositol monophosphatase, IMPase), fixation of atmospheric nitrogen (N2) (e.g. NifH) and defenses against stresses (e.g. chaperones). By using bioinformatic tools, it was possible to attribute probable functions to ten hypothetical proteins. For another ten proteins classified as “NO related COG” group, we analyzed by RT-qPCR the relative expression of their coding-genes in response to the nodulation-gene inducer genistein. Six of these genes were up-regulated, including blr0227, which may be related to polyhydroxybutyrate (PHB) biosynthesis and competitiveness for nodulation.ConclusionsThe proteomic map contributed to the identification of several proteins of B. diazoefficiens under free-living conditions and our approach—combining bioinformatics and gene-expression assays—resulted in new information about unknown genes that might play important roles in the establishment of the symbiosis with soybean.

【 授权许可】

Unknown   
© Gomes et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.