Genetic relationships between clinical and non-clinical strains of Yersinia enterocolitica biovar 1A as revealed by multilocus enzyme electrophoresis and multilocus restriction typing

BMC Microbiology

Research Article

Jugsharan S Virdi¹  Sarita Mallik¹ 

[1] Microbial Pathogenicity Laboratory, Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, 110 021, New Delhi, India;

关键词: Clonal Complex;    Yersinia Enterocolitica;    Clonal Group;    Variable Number Tandem Repeat Analysis;    Multilocus Enzyme Electrophoresis;   

DOI  :  10.1186/1471-2180-10-158

 received in 2009-12-23, accepted in 2010-05-28,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundGenetic relationships among 81 strains of Y. enterocolitica biovar 1A isolated from clinical and non-clinical sources were discerned by multilocus enzyme electrophoresis (MLEE) and multilocus restriction typing (MLRT) using six loci each. Such studies may reveal associations between the genotypes of the strains and their sources of isolation.ResultsAll loci were polymorphic and generated 62 electrophoretic types (ETs) and 12 restriction types (RTs). The mean genetic diversity (H) of the strains by MLEE and MLRT was 0.566 and 0.441 respectively. MLEE (DI = 0.98) was more discriminatory and clustered Y. enterocolitica biovar 1A strains into four groups, while MLRT (DI = 0.77) identified two distinct groups. BURST (Based Upon Related Sequence Types) analysis of the MLRT data suggested aquatic serotype O:6,30-6,31 isolates to be the ancestral strains from which, clinical O:6,30-6,31 strains might have originated by host adaptation and genetic change.ConclusionMLEE revealed greater genetic diversity among strains of Y. enterocolitica biovar 1A and clustered strains in four groups, while MLRT grouped the strains into two groups. BURST analysis of MLRT data nevertheless provided newer insights into the probable evolution of clinical strains from aquatic strains.

【 授权许可】

CC BY   
© Mallik and Virdi; licensee BioMed Central Ltd. 2010