Feasibility and accuracy evaluation of three human papillomavirus assays for FTA card-based sampling: a pilot study in cervical cancer screening

BMC Cancer

Research Article

Fang-Hui Zhao¹  Wei He¹  Shang-Ying Hu¹  Wen Chen¹  You-Lin Qiao¹  Shao-Ming Wang¹  Feng Chen¹  Xin-Ming Ma¹  Yu-Qing Zhang¹  Jian Wang²  Priya Sivasubramaniam³ 

[1] Cancer Institute/Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, P.O. Box 2258, 17 South Panjiayuan Lane, 100021, Beijing, China;Cancer Institute/Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, P.O. Box 2258, 17 South Panjiayuan Lane, 100021, Beijing, China;Beijing Municipal Institute of Labour Protection, 100021, Beijing, China;Cancer Institute/Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, P.O. Box 2258, 17 South Panjiayuan Lane, 100021, Beijing, China;Vanderbilt University School of Medicine, 37212, Nashville, TN, USA;

关键词: Whatman Indicating FTA Elute® card (FTA card);    Cervical cancer screening;    care;    Cobas®4800;    Hybrid capture 2 (HC2);   

DOI  :  10.1186/s12885-015-1882-9

 received in 2015-02-02, accepted in 2015-10-30,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundLiquid-state specimen carriers are inadequate for sample transportation in large-scale screening projects in low-resource settings, which necessitates the exploration of novel non-hazardous solid-state alternatives. Studies investigating the feasibility and accuracy of a solid-state human papillomavirus (HPV) sampling medium in combination with different down-stream HPV DNA assays for cervical cancer screening are needed.MethodsWe collected two cervical specimens from 396 women, aged 25–65 years, who were enrolled in a cervical cancer screening trial. One sample was stored using DCM preservative solution and the other was applied to a Whatman Indicating FTA Elute® card (FTA card). All specimens were processed using three HPV testing methods, including Hybrid capture 2 (HC2), careHPV™, and Cobas®4800 tests. All the women underwent a rigorous colposcopic evaluation that included using a microbiopsy protocol.ResultsCompared to the liquid-based carrier, the FTA card demonstrated comparable sensitivity for detecting high grade Cervical Intraepithelial Neoplasia (CIN) using HC2 (91.7 %), careHPV™ (83.3 %), and Cobas®4800 (91.7 %) tests. Moreover, the FTA card showed a higher specificity compared to a liquid-based carrier for HC2 (79.5 % vs. 71.6 %, P = 0.015), comparable specificity for careHPV™ (78.1 % vs. 73.0 %, P > 0.05), but lower specificity for the Cobas®4800 test (62.4 % vs. 69.9 %, P = 0.032). Generally, the FTA card-based sampling medium’s accuracy was comparable with that of liquid-based medium for the three HPV testing assays.ConclusionsFTA cards are a promising sample carrier for cervical cancer screening. With further optimization, it can be utilized for HPV testing in areas of varying economic development.

【 授权许可】

CC BY   
© Wang et al. 2015