A pilot study to understand feasibility and acceptability of stool and cord blood sample collection for a large-scale longitudinal birth cohort

BMC Pregnancy and Childbirth

Research Article

M. Noursadeghi¹  E. Tsaliki¹  E. M. Riley²  A. J. Rodger³  N. Field³  P. Brocklehurst⁴  S. R. Bailey⁵  C. L. Townsend⁵  C. Mallet⁶  H. Dent⁶  T. D. Lawley⁷ 

[1] Division of Infection and Immunity, University College London, London, UK;Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK;Institute for Global Health, University College London, London, UK;Institute for Women’s Health, University College London, London, UK;Institute of Applied Health Research, University of Birmingham, Birmingham, UK;UCL Institute of Child Health, University College London, London, UK;University College London Hospital, London, UK;Wellcome Trust Sanger Institute, Cambridge, UK;

关键词: Biological samples;    Infant faeces;    Cord blood;    Feasibility;    Acceptability;    Large-scale birth cohorts;    Bioarchive;   

DOI  :  10.1186/s12884-017-1627-7

 received in 2016-03-11, accepted in 2017-12-13,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundFew data are available to guide biological sample collection around the time of birth for large-scale birth cohorts. We are designing a large UK birth cohort to investigate the role of infection and the developing immune system in determining future health and disease. We undertook a pilot to develop methodology for the main study, gain practical experience of collecting samples, and understand the acceptability of sample collection to women in late pregnancy.MethodsBetween February–July 2014, we piloted the feasibility and acceptability of collecting maternal stool, baby stool and cord blood samples from participants recruited at prolonged pregnancy and planned pre-labour caesarean section clinics at University College London Hospital. Participating women were asked to complete acceptability questionnaires.ResultsOverall, 265 women were approached and 171 (65%) participated, with ≥1 sample collected from 113 women or their baby (66%). Women had a mean age of 34 years, were primarily of white ethnicity (130/166, 78%), and half were nulliparous (86/169, 51%). Women undergoing planned pre-labour caesarean section were more likely than those who delivered vaginally to provide ≥1 sample (98% vs 54%), but less likely to provide maternal stool (10% vs 43%). Pre-sample questionnaires were completed by 110/171 women (64%). Most women reported feeling comfortable with samples being collected from their baby (<10% uncomfortable), but were less comfortable about their own stool (19% uncomfortable) or a vaginal swab (24% uncomfortable).ConclusionsIt is possible to collect a range of biological samples from women around the time of delivery, and this was acceptable for most women. These data inform study design and protocol development for large-scale birth cohorts.

【 授权许可】

CC BY   
© The Author(s). 2017