Estimation of divergence time between two sibling species of the Anopheles (Kerteszia) cruziicomplex using a multilocus approach

BMC Evolutionary Biology

Research Article

Carlos J Carvalho-Pinto¹  Camila J Mazzoni²  Alexandre A Peixoto³  Luísa DP Rona³ 

[1] Departamento de Microbiologia e Parasitologia, CCB, Universidade Federal de Santa Catarina, 88040-970, Florianópolis, SC, Brazil;Environmental Research Institute, University College Cork, Cork, Ireland;Evolutionary Genetics Group, Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Str, 17, D-10315, Berlin, Germany;Laboratório de Biologia Molecular de Insetos, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil 4365, 21045-900, Rio de Janeiro, RJ, Brazil;

关键词: Ribosomal Protein;    Effective Population Size;    Sibling Species;    Circadian Gene;    Multilocus Analysis;   

DOI  :  10.1186/1471-2148-10-91

 received in 2009-10-05, accepted in 2010-03-31,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundAnopheles cruzii is the primary human Plasmodium vector in southern and southeastern Brazil. The distribution of this mosquito follows the coast of the Brazilian Atlantic Forest. Previous studies indicated that An. cruzii is a complex of cryptic species.ResultsA multilocus approach using six loci, three circadian clock genes and three encoding ribosomal proteins, was implemented to investigate in more detail the genetic differentiation between the An. cruzii populations from Santa Catarina (southern Brazil) and Bahia States (northeastern Brazil) that represent two sibling species. The analysis revealed very high FSTvalues and fixed differences between the two An. cruzii sibling species in all loci, irrespective of their function. An Isolation with Migration model was fit to the data using the IM program. The results reveal no migration in either direction and allowed a rough estimate of the divergence time between the two sibling species.ConclusionsPopulation genetics analysis of An. cruzii samples from two Brazilian localities using a multilocus approach confirmed that they represent two different sibling species in this complex. The results suggest that the two species have not exchanged migrants since their separation and that they possibly diverged between 1.1 and 3.6 million years ago, a period of intense climatic changes.

【 授权许可】

Unknown   
© Rona et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.