Some ethylene biosynthesis and AP2/ERF genes reveal a specific pattern of expression during somatic embryogenesis in Hevea brasiliensis

BMC Plant Biology

Research Article

Florence Dessailly¹  Ludovic Lardet¹  Jean-François Dufayard¹  Florence Martin¹  Julie Leclercq¹  Pascal Montoro¹  Maryannick Rio¹  Riza-Arief Putranto²  Piyanuch Piyatrakul³ 

[1]CIRAD, UMR AGAP, F-34398, Montpellier, France

[2]CIRAD, UMR AGAP, F-34398, Montpellier, France

[3]Indonesian Biotechnology Research Institute for Estate Crops, Bogor, Indonesia

[4]CIRAD, UMR AGAP, F-34398, Montpellier, France

[5]Rubber Research Institute, 10900, Chatuchak, Bangkok, Thailand

关键词: Gene expression;    Plant hormone;    Plant regeneration;    Recalcitrant;    Rubber;    Signalling;    Transcription factor;   

DOI  :  10.1186/1471-2229-12-244

 received in 2012-07-05, accepted in 2012-11-30,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundEthylene production and signalling play an important role in somatic embryogenesis, especially for species that are recalcitrant in in vitro culture. The AP2/ERF superfamily has been identified and classified in Hevea brasiliensis. This superfamily includes the ERFs involved in response to ethylene. The relative transcript abundance of ethylene biosynthesis genes and of AP2/ERF genes was analysed during somatic embryogenesis for callus lines with different regeneration potential, in order to identify genes regulated during that process.ResultsThe analysis of relative transcript abundance was carried out by real-time RT-PCR for 142 genes. The transcripts of ERFs from group I, VII and VIII were abundant at all stages of the somatic embryogenesis process. Forty genetic expression markers for callus regeneration capacity were identified. Fourteen markers were found for proliferating calli and 35 markers for calli at the end of the embryogenesis induction phase. Sixteen markers discriminated between normal and abnormal embryos and, lastly, there were 36 markers of conversion into plantlets. A phylogenetic analysis comparing the sequences of the AP2 domains of Hevea and Arabidopsis genes enabled us to predict the function of 13 expression marker genes.ConclusionsThis first characterization of the AP2/ERF superfamily in Hevea revealed dramatic regulation of the expression of AP2/ERF genes during the somatic embryogenesis process. The gene expression markers of proliferating callus capacity to regenerate plants by somatic embryogenesis should make it possible to predict callus lines suitable to be used for multiplication. Further functional characterization of these markers opens up prospects for discovering specific AP2/ERF functions in the Hevea species for which somatic embryogenesis is difficult.

【 授权许可】

Unknown   
© Piyatrakul et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.