Local pamidronate influences fracture healing in a rodent femur fracture model: an experimental study

BMC Musculoskeletal Disorders

Research Article

Thomas Pufe¹  Nicolai Purcz²  Tim Klüter³  Deike Varoga³  Stefanie Fitchen-Oestern³  Stefan Brüggemann³  Andreas Seekamp³  Peter Behrendt³  Matthias Weuster³  Leif Menzdorf³  Sebastian Lippross³  Claus C Glueer⁴ 

[1] Department of Anatomy and Cell Biology, RWTH Aachen University, Aachen, Germany;Department of Oral and Maxillofacial Surgery, University Medical Center of Schleswig-Holstein, Campus Kiel, Kiel, Germany;Department of Trauma Surgery, University Medical Center of Schleswig-Holstein, Campus Kiel, Kiel, Germany;Section of Biomedical Imaging, Department of Radiology and Neuroradiology, University Medical Center of Schleswig-Holstein, Campus Kiel, Kiel, Germany;

关键词: Bisphosphonates;    Zoledronic Acid;    Osteonecrosis;    Fracture Healing;    Bone Volume;   

DOI  :  10.1186/s12891-016-1113-9

 received in 2015-12-02, accepted in 2016-06-02,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundBisphosphonates are a main component in the therapy of osteoporosis and other bone resorptive diseases. Previous studies have shown a positive effect of systemically applied bisphosphonates on fracture healing. Nevertheless high doses are related to side effects like osteonecrosis of the jaw, nephrotoxis and gastrointestinal symptoms. In this study we investigated the effect of locally applied pamidronate on fracture healing.MethodsIn a rodent model a simple femur fracture was set in female Wistar rats. We performed intramedullary fixation of the fracture and placed a collagen matrix around the fracture area. One group was treated with pamidronate, the other group with placebo via the matrix.To investigate the volume and quality of the callus we used micro-CT (μCT) and histology after 14 and 28 days.ResultsOur results show a positive influence of local applied pamidronate on callus volume. After 14 days an insignificant increase of callus volume in the treated animals was seen. 28 days after trauma the increase of callus volume in the treatment group was significantly higher in comparison to the control group. Osteonecrosis was not seen.ConclusionsLocally applied bisphosphonates increase the callus volume in fracture healing.

【 授权许可】

CC BY   
© The Author(s). 2016