MB3W1 is an orthotopic xenograft model for anaplastic medulloblastoma displaying cancer stem cell- and Group 3-properties

BMC Cancer

Technical Advance

Jürgen Krauss¹  Giles H. Vince¹  Tilmann Schweitzer¹  Daniel Picard²  Marc Remke³  Stefan Rutkowski⁴  Andre O. von Bueren⁵  Susanne Dietl⁶  Michael D. Taylor⁷  Vijay Ramaswamy⁷  Andreas Rosenwald⁸  Camelia M. Monoranu⁸  Andreas Beilhack⁹  Simone Riedel⁹  Matthias Wölfl^1,10  Sebastian Dietl^1,10  Frank Deinlein^1,10  Matthias Eyrich^1,10  Stefanie Schwinn^1,10  Paul G. Schlegel^1,11 

[1] Department of Neurosurgery, University of Würzburg, Würzburg, Germany;Department of Pediatric Oncology, Hematology and Clinical Immunology / Department of Neuropathology, Heinrich Heine University, Düsseldorf, Germany;Department of Pediatric Oncology, Hematology and Clinical Immunology / Department of Neuropathology, Heinrich Heine University, Düsseldorf, Germany;Division of Neurosurgery, Arthur and Sonia Labatt Brain Tumor Research Centre, Hospital for Sick Children, Toronto, Canada;Department of Pediatric Oncology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany;Department of Pediatrics and Adolescent Medicine, Division of Pediatric Hematology and Oncology, University Hospital of Geneva, Geneva, Switzerland;Department of Surgery II, University of Würzburg, Würzburg, Germany;Division of Neurosurgery, Arthur and Sonia Labatt Brain Tumor Research Centre, Hospital for Sick Children, Toronto, Canada;Institute of Pathology, University of Würzburg, Würzburg, Germany;Interdisciplinary Center for Clinical Research Laboratory (IZKF Würzburg), Department of Internal Medicine II, University of Würzburg, Würzburg, Germany;University Children’s Hospital, Pediatric Oncology, Hematology and Stem Cell Transplantation, University of Würzburg, Würzburg, Germany;University Children’s Hospital, Pediatric Oncology, Hematology and Stem Cell Transplantation, University of Würzburg, Würzburg, Germany;Comprehensive Cancer Center Mainfranken, University of Würzburg, Würzburg, Germany;

关键词: Anaplastic medulloblastoma;    Group 3;    Orthotopic xenograft;    Cancer stem cells;    Animal model;    Brain tumor;    Children;   

DOI  :  10.1186/s12885-016-2170-z

 received in 2015-05-25, accepted in 2016-02-14,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundMedulloblastoma is the most common malignant brain tumor in children and can be divided in different molecular subgroups. Patients whose tumor is classified as a Group 3 tumor have a dismal prognosis. However only very few tumor models are available for this subgroup.MethodsWe established a robust orthotopic xenograft model with a cell line derived from the malignant pleural effusions of a child suffering from a Group 3 medulloblastoma.ResultsBesides classical characteristics of this tumor subgroup, the cells display cancer stem cell characteristics including neurosphere formation, multilineage differentiation, CD133/CD15 expression, high ALDH-activity and high tumorigenicity in immunocompromised mice with xenografts exactly recapitulating the original tumor architecture.ConclusionsThis model using unmanipulated, human medulloblastoma cells will enable translational research, specifically focused on Group 3 medulloblastoma.

【 授权许可】

CC BY   
© Dietl et al. 2016