RNA-seq analysis of the influence of anaerobiosis and FNR on Shigella flexneri

BMC Genomics

Research Article

Marta Vergara-Irigaray¹  Christoph M Tang¹  Maria C Fookes²  Nicholas R Thomson² 

[1] Sir William Dunn School of Pathology, Oxford University, Oxford, United Kingdom;Centre for Molecular Microbiology and Infection, Imperial College London, London, United Kingdom;The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, United Kingdom;

关键词: Anaerobic Condition;    Enteric Pathogen;    Shigella Flexneri;    Virulence Plasmid;    Host Cell Invasion;   

DOI  :  10.1186/1471-2164-15-438

 received in 2013-09-19, accepted in 2014-05-23,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundShigella flexneri is an important human pathogen that has to adapt to the anaerobic environment in the gastrointestinal tract to cause dysentery. To define the influence of anaerobiosis on the virulence of Shigella, we performed deep RNA sequencing to identify transcriptomic differences that are induced by anaerobiosis and modulated by the anaerobic Fumarate and Nitrate Reduction regulator, FNR.ResultsWe found that 528 chromosomal genes were differentially expressed in response to anaerobic conditions; of these, 228 genes were also influenced by FNR. Genes that were up-regulated in anaerobic conditions are involved in carbon transport and metabolism (e.g. ptsG, manX, murQ, cysP, cra), DNA topology and regulation (e.g. ygiP, stpA, hns), host interactions (e.g. yciD, nmpC, slyB, gapA, shf, msbB) and survival within the gastrointestinal tract (e.g. shiA, ospI, adiY, cysP). Interestingly, there was a marked effect of available oxygen on genes involved in Type III secretion system (T3SS), which is required for host cell invasion and pathogenesis. These genes, located on the large Shigella virulence plasmid, were down regulated in anaerobiosis in an FNR-dependent manner. We also confirmed anaerobic induction of csrB and csrC small RNAs in an FNR-independent manner.ConclusionsAnaerobiosis promotes survival and adaption strategies of Shigella, while modulating virulence plasmid genes involved in T3SS-mediated host cell invasion. The influence of FNR on this process is more extensive than previously appreciated, although aside from the virulence plasmid, this transcriptional regulator does not govern expression of genes on other horizontally acquired sequences on the chromosome such as pathogenicity islands.

【 授权许可】

Unknown   
© Vergara-Irigaray et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.