Distribution of short interstitial telomere motifs in two plant genomes: putative origin and function

BMC Plant Biology

Research Article

Jean-François Rami¹  Christine Gaspin²  Bernard Lescure³ 

[1] Centre de coopération internationale en recherche agronomique pour le développement (CIRAD), UMR Développement et Amélioration des Plantes, TA A96/3, Avenue Agropolis, 34398, Montpellier, Cedex 5, France;INRA Toulouse, UBIA & Plateforme Bioinformatique, UR 875, Chemin de Borde Rouge, Auzeville BP 52627, 31326, Castanet-Tolosan, France;Laboratoire Interactions Plantes-Microorganismes (LIPM), UMR 441-2594 (INRA-CNRS), BP 52627, Chemin de Borde Rouge, Auzeville BP 52627, 31326, Castanet-Tolosan, France;

关键词: Telomere Repeat;    Ribosome Biogenesis;    snoRNA Gene;    Proliferate Cellular Nuclear Antigen;    Sativa Genome;   

DOI  :  10.1186/1471-2229-10-283

 received in 2009-12-23, accepted in 2010-12-20,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundShort interstitial telomere motifs (telo boxes) are short sequences identical to plant telomere repeat units. They are observed within the 5' region of several genes over-expressed in cycling cells. In synergy with various cis-acting elements, these motifs participate in the activation of expression. Here, we have analysed the distribution of telo boxes within Arabidopsis thaliana and Oryza sativa genomes and their association with genes involved in the biogenesis of the translational apparatus.ResultsOur analysis showed that the distribution of the telo box (AAACCCTA) in different genomic regions of A. thaliana and O. sativa is not random. As is also the case for plant microsatellites, they are preferentially located in the 5' flanking regions of genes, mainly within the 5' UTR, and distributed as a gradient along the direction of transcription. As previously reported in Arabidopsis, a conserved topological association of telo boxes with site II or TEF cis-acting elements is observed in almost all promoters of genes encoding ribosomal proteins in O. sativa. Such a conserved promoter organization can be found in other genes involved in the biogenesis of the translational machinery including rRNA processing proteins and snoRNAs. Strikingly, the association of telo boxes with site II motifs or TEF boxes is conserved in promoters of genes harbouring snoRNA clusters nested within an intron as well as in the 5' flanking regions of non-intronic snoRNA genes. Thus, the search for associations between telo boxes and site II motifs or TEF box in plant genomes could provide a useful tool for characterizing new cryptic RNA pol II promoters.ConclusionsThe data reported in this work support the model previously proposed for the spreading of telo boxes within plant genomes and provide new insights into a putative process for the acquisition of microsatellites in plants. The association of telo boxes with site II or TEF cis-acting elements appears to be an essential feature of plant genes involved in the biogenesis of ribosomes and clearly indicates that most plant snoRNAs are RNA pol II products.

【 授权许可】

CC BY   
© Gaspin et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.