Isolation and characterization of a new naturally immortalized human breast carcinoma cell line, KAIMRC1

BMC Cancer

Research Article

Mohamed Al Balwi¹  Abdul Latif Khan¹  Lolwah Abdullah Alriyees²  Manal Alzaid²  Ahmed Al Askar³  Sabhi Rahman⁴  Nosaibah Samman⁴  Mohamed Boudjelal⁴  Hajar Al Zahrani⁴  Atef Nehdi⁴  Rizwan Ali⁴ 

[1] Department of Pathology and Laboratory Medicine, King Abdullah Medical City (KAMC), NGHA, 11426, Riyadh, Saudi Arabia;Department of Surgery, KAMC, NGHA, 11426, Riyadh, Saudi Arabia;King Abdullah International Medical Research Center/ King Saud bin Abdulaziz University for Health Sciences (KSAU-HS), King Abdulaziz Medical City (KAMC), NGHA, 11426, Riyadh, Saudi Arabia;Medical Research Core Facility and Platforms (MRCFP), King Abdullah International Medical Research Center (KAIMRC), National Guard Health Affairs (NGHA), P.O. Box 22490, 11426, Riyadh, Saudi Arabia;King Abdullah International Medical Research Center/ King Saud bin Abdulaziz University for Health Sciences (KSAU-HS), King Abdulaziz Medical City (KAMC), NGHA, 11426, Riyadh, Saudi Arabia;

关键词: Breast cancer;    Characterization;    Isolation;    Drug treatment;    Gene expression;    Cell line;    Natural transformation;    Immortalization;    BRCA1;    BRCA2;   

DOI  :  10.1186/s12885-017-3812-5

 received in 2017-03-14, accepted in 2017-11-21,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundBreast cancer is one of the most common cancer and a leading cause of death in women. Up to date the most commonly used breast cancer cell lines are originating from Caucasians or Afro-Americans but rarely cells are being derived from other ethnic groups. Here we describe for the first time the establishment of a naturally transformed breast cancer cell line, KAIMRC1 from an Arab woman of age 62 suffering from stage IIB breast cancer (T2N1M0). Moreover, we have characterized these cells for the biological and molecular markers, induction of MAPK pathways as well as its response to different commercially available drugs and compounds.MethodsBreast cancer tissue sections were minced and cultured in media for several weeks. KAIMRC1 cells were successfully isolated from one of the primary breast tumor tissue cultures without any enzymatic digestion. To study the growth characteristics of the cells, wound healing assay, clonogenic assay, cell proliferation assays and live cell time-lapse microscopy was performed. Karyotyping, Immunophenotyping and molecular pathway specific compound treatment was also performed. A selective breast cancer gene expression panel was used to identify genes involved in the signal transduction dysregulation and malfunction of normal biological processes during breast carcinogenesis.ResultsThese cells are ER/PR-positive and HER2-negative. The epithelial nature of these cells was confirmed by flow cytometry analysis using epithelial cell markers. They are cuboidal in shape and relatively smaller in size as compared to established cell lines, MCF-7, MDA MB-231 and the normal breast cell line, MCF-10A. In normal cell culture conditions these cells showed the capability of growing both in monolayer as well as in 3-D conformation. They showed a doubling time in vitro of approximately 24 h. They exhibit a modal karyotype of 58–63,X with abnormalities in a couple of chromosomes. KAIMRC1 cells were found to be more responsive to drug treatment in vitro in comparison to the established MDA MB-231 and MCF-7 cell lines.ConclusionsIn conclusion we have isolated and characterized a new naturally immortalized breast cell line, KAIMRC1 with a potential to play a key role in opening up novel avenues towards the understanding of breast carcinoma.

【 授权许可】

CC BY   
© The Author(s). 2017