Bacterial isolates and their antimicrobial susceptibility pattern among patients with external ocular infections at Borumeda hospital, Northeast Ethiopia

BMC Ophthalmology

Research Article

Zelalem Addis¹  Baye Gelaw¹  Abate Assefa¹  Yared Assefa²  Birtukan Shiferaw³ 

[1] Department of Medical Microbiology, School of Biomedical and Laboratory sciences, CMHS, UOG, P.O. box 196, Gondar, Ethiopia;Department of Ophthalmology, School of Medicine, CMHS, UOG, P.O. box 196, Gondar, Ethiopia;School of Biomedical and Laboratory sciences, College of Medicine and Health Sciences (CMHS), University of Gondar (UOG), P.O. box 196, Gondar, Ethiopia;

关键词: Bacterial isolate;    External ocular infections;    Drug susceptibility pattern;   

DOI  :  10.1186/s12886-015-0078-z

 received in 2014-10-28, accepted in 2015-07-16,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundBacteria are major cause of ocular infections and possible loss of vision. The emergence of antimicrobial resistant bacteria increases the risk of treatment failure with potentially serious consequences. The aim of this study was to determine the prevalence of bacterial isolates and their antimicrobial susceptibility pattern among patients with external ocular infections.MethodsA cross sectional study was conducted among 160 patients with external ocular infections at Borumeda hospital, Northeast Ethiopia. Socio-demographic and clinical data were collected using structured questionnaire. External ocular specimens were collected using sterile swabs and inoculated on MacConkey agar, Chocolate agar and Blood agar culture Medias. Presumptive isolates were further identified by a series of biochemical tests. The antimicrobial susceptibility patterns of the isolates were determined by disk diffusion method.ResultThe overall prevalence of bacterial pathogens among external ocular samples was 59.4 %. The majority of the isolates (93.7 %; 89/95) were Gram positive and the other 6.3 % (6/95) Gram negative bacteria. The proportion of coagulase negative Staphylococci among the Gram positive bacterial isolates was 53.7 % (n = 51/95). All Gram positive isolates were susceptible for vancomycin but 67.4 % (n = 60/95) of them were resistant against amoxicillin. Moreover, drug resistance to tetracycline, norfloxacylin, ceftriaxone and ciprofloxacin were observed among Gram negative bacteria isolates.ConclusionThe prevalence of bacterial pathogens among external ocular samples was high and the predominant isolate was coagulase negative Staphylococci. Exceptionally high amoxicillin resistance was observed among Gram positive bacterial isolates that may dictate to conduct drug susceptibility test routinely.

【 授权许可】

CC BY   
© Shiferaw et al. 2015