High glucose increases LPS-induced DC apoptosis through modulation of ERK1/2, AKT and Bax/Bcl-2

BMC Gastroenterology

Research Article

Mei Feng¹  Jie Zhang¹  Yueran Zhao¹  Chunyan Ma¹  Yulian Jiao¹  Ling Gao¹  Ying Ju²  Qiuying Sun³  Juan Li⁴  Yan Wang⁵  Jun Wang⁶ 

[1] Central Laboratory, Shandong Provincial Hospital affiliated to Shandong University, Jinan, China;Department of Clinical Laboratory, Shandong Provincial Hospital affiliated to Shandong University, Jinan, China;Department of Endocrinology, Shandong Provincial Hospital affiliated to Shandong University, Jinan, China;Department of Obstetrics and Gynecology, Shandong Provincial Hospital affiliated to Shandong University, Jinan, China;Department of Occupational Environmental Health Monitoring and Evaluation, Shandong Center for Disease Control and Prevention, Jinan, China;Jinan Central Hospital, Affiliated with Shandong University, Jinan, China;

关键词: Dendritic cell;    Apoptosis;    Diabetes mellitus;    Glucose;    Gastrointestinal complications diabetes;   

DOI  :  10.1186/1471-230X-14-98

 received in 2013-12-27, accepted in 2014-05-14,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThis study investigates the effect of glucose on the LPS-induced apoptosis of dendritic cells in the intestinal tract of mice and the dendritic cell line DC2.4.MethodsFlow cytometry was used to detect dendritic cell apoptosis both in vivo and in vitro. Hoechst 33258 staining was used to detect the morphological changes characteristic of apoptotic nuclei. Expression of apoptosis related proteins was investigated by western blot analysis and immunohistochemistry.ResultsPretreatment with a high concentration of glucose increased apoptosis of LPS-treated dendritic cells both in vivo and in vitro at 24 h. No effect was evident at the earlier time points of 15 min and 6 h in vitro. Furthermore, at 24 hours the expression of the survival proteins AKT, ERK and Bcl-2 was decreased, while the expression of the proapoptotic protein Bax was increased. AKT, ERK, Bcl-2 and Bax were mainly located in the cytoplasm by immunohistochemistry.ConclusionsThese results suggest that high glucose concentrations might prime dendritic cells for apoptosis induced by LPS in the intestinal tract through upregulating the expression of Bax and downregulating the expression of AKT, ERK and Bcl-2. Therefore, this study may give clues to understanding the immunological mechanism behind gastrointestinal complications in diabetes mellitus.

【 授权许可】

Unknown   
© Feng et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.