Ardipusilloside I induces apoptosis by regulating Bcl-2 family proteins in human mucoepidermoid carcinoma Mc3 cells

BMC Complementary and Alternative Medicine

Research Article

Song Jin¹  Rong Wang²  Tao-Li Zhang²  Xiao-Juan Wang²  Xin Xiao²  Wei-Dong Zhang²  Peng-Yuan Wang²  Xiao-Fang Xu³ 

[1] Department of Oral and Maxillofacial Surgery, The 22nd hospital of PLA, Middle-Yanqiao Road, 816000, Ge’ermu, Qinghai Province, China;Department of Pharmaceutical Preparation, School of Stomatology, The Fourth Military Medical University, 145 Chang Le Xi Road, 710032, Xi’an, Shaanxi Province, China;Department of Stomatology, 307 Hospital of PLA, 100071, Beijing, China;Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical University, 145 Chang Le Xi Road, 710032, Xi’an, Shaanxi Province, China;

关键词: Mucoepidermoid carcinoma;    Mc3 cell line;    Apoptosis;    Bcl-2;    Traditional Chinese herb;    Ardipusilloside I;   

DOI  :  10.1186/1472-6882-13-322

 received in 2012-05-17, accepted in 2013-11-15,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundArdisia pusilla A. DC., family Myrsinaceae, is a traditional Chinese medicine named Jiu Jie Long with a variety of pharmacological functions including anti-cancer activities. In this study, we purified a natural triterpenoid saponin, ardipusilloside I, from Ardisia pusilla, and show that it exhibits inhibitory activities in human mucoepidermoid carcinoma Mc3 cells. We also investigated the underlying mechanisms of proliferation inhibition that ardipusilloside I exerts on Mc3 cells.MethodsMTT test was used to detect cell proliferation. Cell apoptosis was detected by transmission electron microscopy, Hoechst-33342 staining, DNA fragmentation detection, and flow cytometry. We also used western blot analysis to detect the potential mechanisms of apoptosis.ResultsArdipusilloside I affected the viability of Mc3 cells in a dose- and time-dependent manner. The IC50 of ardipusilloside I was approximately 9.98 μg/ml at 48 h of treatment. Characteristic morphological changes of apoptosis, including nuclear condensation, boundary aggregation and splitting, and DNA fragmentation, were seen after treatment with 10 μg/ml ardipusilloside I for 48 h. Western blots demonstrated that ardipusilloside I caused Mc3 cell death through the induction of apoptosis by downregulation of Bcl-2 protein levels and upregulation of Bax and caspase-3 protein levels.ConclusionsOur results revealed that ardipusilloside I could be a new active substance for mucoepidermoid carcinoma treatment. We demonstrated that the potential mechanism of inhibition might be through the induction of apoptosis by regulation of Bcl-2 family protein levels. This suggests a further rationale for the development of ardipusilloside I as an anti-cancer agent.

【 授权许可】

Unknown   
© Xu et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.