Inhibitory effect of Samul-tang on retinal neovascularization in oxygen-induced retinopathy

BMC Complementary and Alternative Medicine

Research Article

Junghyun Kim¹  Eun Jin Sohn¹  Jin Sook Kim¹  Yun Mi Lee¹  Kyuhyung Jo¹  Chan-Sik Kim¹ 

[1] Korean Medicine-Based Herbal Drug Development Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine (KIOM), 1672 Yuseongdaero, Yuseong-gu, 305-811, Daejeon, South Korea;

关键词: Retinal neovascularization;    Stromal cell-derived factor 1;    Chemokine (C-X-C motif) receptor 4;    Vascular endothelial growth factor;    Oxygen-induced retinopathy;   

DOI  :  10.1186/s12906-015-0800-7

 received in 2015-02-05, accepted in 2015-08-03,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundRetinal neovascularization is a common cause of vision loss in proliferative diabetic retinopathy, retinopathy of prematurity and age-related macular degeneration. Samul-tang (SMT) is a widely used traditional herbal medicine in East Asia and is also known as Shimotsu-to in Japanese and Si-Wu decoction in Chinese. This study was designed to evaluate the inhibitory effect of SMT on retinal pathogenic angiogenesis in a mouse model of oxygen-induced retinopathy (OIR).MethodThe mice were exposed to a 75 % concentration of oxygen for five days, starting on postnatal day 7 (P7-P12). The mice were then exposed to room air and were intraperitoneally injected with SMT (10 mg/kg or 50 mg/kg) once per day for five days (P12-P16). On P17, we measured retinal neovascularization and evaluated both the expression of angiogenesis-related proteins and changes in the gene expression level in the mRNA.ResultsSMT reduced the area of the central retina and reduced retinal neovascularization in OIR mice. The protein array revealed that SMT reduced the level of SDF-1 protein expression. Quantitative real-time PCR revealed that the HIF-1α, SDF-1, CXCR4 and VEGF mRNA levels in the retinas of OIR mice were elevated compared with those of normal control mice. However, SMT decreased the levels of HIF-1α, SDF-1, CXCR4 and VEGF mRNA in OIR mice.ConclusionWe are the first to elucidate that SMT inhibits the retinal pathogenic angiogenesis induced by ischemic retinopathy in OIR mice. SMT significantly inhibited retinal neovascularization by downregulating HIF-1α, SDF-1, CXCR4 and VEGF. Based on the results of our study, SMT could be a useful herbal medicine for treating ischemic retinopathy.

【 授权许可】

CC BY   
© Lee et al. 2015

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